Coated capillary tube for the controlled release of reagent

• US 5,387,526 A
• Filed: 03/30/1993
• Issued: 02/07/1995
• Est. Priority Date: 09/11/1990
• Status: Expired due to Fees

• Current Assignee: Vorhies Technologies Inc.
• Original Assignee: General Atomics, Inc.
• Inventors: Garner, Harold R., Tuason, Orenda F., Peranich, Larry S.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): REDDING, DAVID A
• Application Number:
  US08/030,445
• Time in Patent Office: 679 Days
• Field of Search: 422/55-58, 422/82.05, 422/82.08, 422/99, 422/100, 422/102, 435/288, 435/291, 435/6, 436/527, 436/169, 436/810, 436/518, 436/524, 436/94, 356/244, 356/246, 427/2, 427/8, 427/162, 427/164
• US Class Current: 436/169
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2035/00237 Handling microquantities of analyte, e.g. microvalves, capillary networks
• CPC Class Codes: G01N 2035/1062 for testing the liquid while it is in the transfer device
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/29 using visual detection G01N21/31 takes precedence
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

A hollow, elongated, micropipette, which is specially adapted for use in spectrometers and which has an inner wall on which a coating containing a reagent has been deposited, is provided. The reagent is selected from among those that interact with one or more compounds in a sample solution, which is introduced into the micropipette, in order to permit the compounds to be detected by virtue of light absorption or emission by the complexes formed upon interaction of the reagent with the compound of interest in the sample.

Upon introduction of the sample solution into the micropipette, a sufficient amount of the reagent in the coating dissolves in the solution and reacts, either directly or indirectly with a compound or compounds of interest in the solution to render such compound detectable and to permit quantification of the concentration of the compound in the sample. The resulting solution is held in the micropipette for analysis of the light absorption or light emission characteristics of the solution to determine the composition of the solution or the concentration of a particular constituent of the solution.

In preferred embodiments, the coating on the inner surface of the micropipette includes a binding agent or adhesive that delays, preferably for about 5 to 15 sec, the dissolution of the reagent in the solution.

Claims

1. A container, which is constructed so as to be mounted in a spectrometer and which is used for detecting the presence of or quantifying the amount of a biopolymer or biopolymers in a sample solution that is introduced into the container, comprising:

a micropipette or capillary tube that is transparent to at least a portion of the electromagnetic spectrum emitted and detected by a spectrometer and that is constructed so as to be mounted in the spectrometer whereby light passes through the walls of said tube; and

a coating composition deposited on the inner wall of said tube, wherein;

the coating contains one or more reagents and a binding agent;

wherein the binding agent delays distribution of the reagent in the sample solution for at least about 5 seconds to permit uniform dissolution upon filling, and which said binding agent contains one or more ingredients selected from the group consisting of a sugar, a polysaccharide, a gelatin, a polyacrylamide, and agarose,

the reagent specifically interacts with one or more biopolymers in the sample solution to form complexes, whereby, upon exposure to electromagnetic radiation emitted by the spectrometer through the walls of the tube, the complexes absorb or emit electromagnetic radiation; and

the coating contains a sufficient amount of the reagent(s) in the coating to permit detection of the complexes by the spectrometer.

2. The container of claim 1, wherein the reagent is a molecule which specifically interacts with a biopolymer selected from the group consisting of DNA and RNA to form a complex that absorbs, emits or produces electromagnetic radiation of a particular wavelength to detect, identify, and quantitate the concentration of the biopolymer.

3. The container of claim 1, wherein the binding agent is sucrose, gelatin or a mixture of sucrose and gelatin.

4. The container of claim 1, wherein said binding agent is deposited on said wall between said reagent and said wall.

5. The container of claim 1, wherein said binding agent and reagent are mixed prior to deposition on said wall.

6. A method for analyzing a sample solution, comprising:

introducing the solution into a container of claim 1;

directing incident electromagnetic radiation into the container; and

detecting electromagnetic radiation that is transmitted from or through the container, wherein the container is transparent to both the incident and transmitted electromagnetic radiation.

7. The method of claim 6, wherein the reagent is a molecule which specifically interacts with a biopolymer selected from the group consisting of DNA and RNA to form a complex that absorbs, emits or produces electromagnetic radiation of a particular wavelength to detect, identify, and quantitate the concentration of the biopolymer.

8. A method for uniformly coating a reagent and a binding agent onto the inner wall of a capillary tube which is constructed so as to be mounted in a spectrometer and can be used for quantifying the amount of a biopolymer or biopolymers in a sample solution that is introduced into the capillary tube, because the reagent will specifically interact with one or more biopolymers in the sample solution to form complexes, which complexes upon exposure to electromagnetic radiation emitted by the spectrometer through the walls of the tube, absorb or emit electromagnetic radiation,

which method comprises:

introducing a coating mixture in the form of a solution that includes a binding agent and at least one reagent into one end of the tube;

drawing said coating mixture through the tube by applying a partial vacuum to the opposite end and removing excess coating mixture therethrough; and

subjecting the container to evaporating conditions to evaporate and remove the solvent whereby a uniform coating composition of said reagent attached by said binding agent is formed on the inner wall of the capillary tube.

9. The method of claim 8, wherein removal of said solvent is effected by the flow of air by applying suction or pressure.

10. The method of claim 8, wherein the outside diameter of said capillary tube is not greater than about 0.7 mm.

11. A container, which is constructed so as to be mounted in a spectrometer and which is used for detecting the presence of or quantifying the amount of a biopolymer or biopolymers in a sample solution that is introduced into the container, comprising:

a micropipette or capillary tube that is transparent to at least a portion of the electromagnetic spectrum emitted and detected by a spectrometer and that is constructed so as to be mounted in the spectrometer whereby light passes through the walls of said tube; and

a coating composition deposited on the inner walls of said tube, wherein;

the coating contains a plurality of reagents that specifically interact with one or more compounds in the sample solution to form complexes that emit or absorb electromagnetic radiation; and

each reagent is deposited in a discrete and separate location on the wall and remains bound to the wall upon introduction of the sample solution.