Electrochemical detection method and apparatus therefor
First Claim
1. An electrochemical detection method using an apparatus includinga first vessel and a second vessel,a reference electrolytic solution filled in said second vessel,first and second closely spaced working electrodes immersed in a sample solution for measurement filled in said first vessel,a stripping electrode immersed in the reference electrolytic solution, andan ionic conductor, arranged between said first vessel and said second vessel, for electrically connecting the sample solution for measurement and the reference electrolytic solution, whereinthe reference electrolytic solution contains an electrolyte which can be deposited and dissolved by an electrochemical reaction by applying a potential to said stripping electrode,in a first stage, a pre-electrolysis potential is applied to said first working electrode with said stripping electrode being connected to said second working electrode to deposit the electrolyte in the reference electrolytic solution on said stripping electrode, andin a second stage, after said second working electrode is disconnected from said stripping electrode, the potential of said stripping electrode is swept to measure a current generated when the electrolyte deposited on said stripping electrode in the first stage is dissolved from said stripping electrode, and the concentration of an analyte in the sample solution for measurement is determined from the measured stripping current.
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Abstract
In an electrochemical detection method using an apparatus including a first vessel and a second vessel, a reference electrolytic solution in the second vessel, first and second closely spaced working electrodes immersed in a sample solution for measurement in the first vessel, a stripping electrode immersed in the reference electrolytic solution, and an ionic conductor arranged between the first vessel and the second vessel, the reference electrolytic solution contains an electrolyte which can be deposited and dissolved by an electrochemical reaction by applying a potential to the stripping electrode. In a first stage, a pre-electrolysis potential is applied to the first working electrode with the stripping electrode being connected to the second working electrode to deposit the electrolyte in the reference electrolytic solution on the stripping electrode. In a second stage, after the second working electrode is disconnected from the stripping electrode, the potential of the stripping electrode is swept to measure a current generated when the electrolyte deposited on the stripping electrode in the first stage is dissolved from the stripping electrode, and the concentration of an analyte exiting in the sample solution for measurement is determined from the measured stripping current.
165 Citations
13 Claims
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1. An electrochemical detection method using an apparatus including
a first vessel and a second vessel, a reference electrolytic solution filled in said second vessel, first and second closely spaced working electrodes immersed in a sample solution for measurement filled in said first vessel, a stripping electrode immersed in the reference electrolytic solution, and an ionic conductor, arranged between said first vessel and said second vessel, for electrically connecting the sample solution for measurement and the reference electrolytic solution, wherein the reference electrolytic solution contains an electrolyte which can be deposited and dissolved by an electrochemical reaction by applying a potential to said stripping electrode, in a first stage, a pre-electrolysis potential is applied to said first working electrode with said stripping electrode being connected to said second working electrode to deposit the electrolyte in the reference electrolytic solution on said stripping electrode, and in a second stage, after said second working electrode is disconnected from said stripping electrode, the potential of said stripping electrode is swept to measure a current generated when the electrolyte deposited on said stripping electrode in the first stage is dissolved from said stripping electrode, and the concentration of an analyte in the sample solution for measurement is determined from the measured stripping current.
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6. An electrochemical detection apparatus including
a first vessel and a second vessel, a reference electrolytic solution filled in said second vessel, first and second closely spaced working electrodes immersed in a sample solution for measurement filled in said first vessel, a stripping electrode immersed in the reference electrolytic solution, and an ionic conductor, arranged between said first vessel and said second vessel, for electrically connecting the sample solution for measurement and the reference electrolytic solution, wherein the reference electrolytic solution contains an electrolyte which can be deposited and dissolved by an electrochemical reaction by applying a potential to said stripping electrode, and said apparatus comprises means for, in a first stage, applying a pre-electrolysis potential to said first working electrode with said stripping electrode being connected to said second working electrode to deposit the electrolyte in the reference electrolytic solution on said stripping electrode, means for, in a second stage, disconnecting said second working electrode from said stripping electrode, means for, in the second stage, sweeping a potential of said stripping electrode after said second working electrode is disconnected from said stripping electrode, and means for, in the second stage, measuring a current generated when the electrolyte deposited on said stripping electrode in the first stage is dissolved from said stripping electrode.
Specification