Biosensor containing a biochemical substance immobilized on a layer of an olefinic-unsaturated, epoxyfunctional polyether
First Claim
1. A biosensor with a selective detection system comprising a polymer and a biochemical substance wherein the detection system is produced in the following manner:
- applying an olefinic-unsaturated, epoxyfunctional polyether to a carrier material in the form of a layer,cross-linking the polyether by means of high-energy radiation to form an epoxyfunctional polymer matrix,treating the layer with an aqueous solution of the biochemical substance, whereby the biochemical substance is immobilized in the polymer matrix by reaction with epoxy groups, andstabilizing the layer by reaction of non-reacted epoxy groups with a compound containing an amino group, a carboxyl group or an amino group and a carboxyl group,wherein the polyether has the following structure;
##STR12## where the following applies;
##STR13## where R3 =--(CH2)m --, with m=1 to 10R4 =H or CH3 ;
R1 = ##STR14## with u=0 to 150, --(CH2)o --, with o=0 to 18, orCH2 --O--R5 --O--CH2 --,whereR5 =--(CH2)p --, ##STR15## with p=2 to 20, q=2 to 4, r=1 to 50,s=0 to 50, t=0 to 25,Ar= ##STR16## R2 =--(CH2 --CH═
CH--CH2)n --, --R6 --, --R6 --O--CO--R7 --CO--O--R6 -- or ##STR17## where n=1 to 50, u=0 to 150,R6 has the same meaning as R5,except that ##STR18## are excluded, and R7 has the following meaning;
##STR19## with q=2 to 4, s=0 to 50, t=0 to 25,v=0 to 20, andAr has the meaning indicated above.
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Abstract
A biosensor is prepared having a selective detection system containing a biochemical substance such as an enzyme immobilized by reaction with epoxy groups of an olefinic-unsaturated, epoxyfunctional polyether. Prior to immobilization, the polyether is applied to a carrier and crosslinked by treatment with high-energy radiation or peroxide to form a layer. After reacting the biochemical substance with epoxy groups, non-reacted epoxy groups are reacted with a compound containing an amino group and/or a carboxyl group such as an amino acid. Before immobilizing of the biochemical substance and after crosslinking, the polyether may be hydrophilized by reacting some of the epoxy groups with a hydrophilic compound such as an amino acid.
190 Citations
12 Claims
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1. A biosensor with a selective detection system comprising a polymer and a biochemical substance wherein the detection system is produced in the following manner:
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applying an olefinic-unsaturated, epoxyfunctional polyether to a carrier material in the form of a layer, cross-linking the polyether by means of high-energy radiation to form an epoxyfunctional polymer matrix, treating the layer with an aqueous solution of the biochemical substance, whereby the biochemical substance is immobilized in the polymer matrix by reaction with epoxy groups, and stabilizing the layer by reaction of non-reacted epoxy groups with a compound containing an amino group, a carboxyl group or an amino group and a carboxyl group, wherein the polyether has the following structure;
##STR12## where the following applies;
##STR13## where R3 =--(CH2)m --, with m=1 to 10R4 =H or CH3 ; R1 = ##STR14## with u=0 to 150, --(CH2)o --, with o=0 to 18, or CH2 --O--R5 --O--CH2 --, where R5 =--(CH2)p --, ##STR15## with p=2 to 20, q=2 to 4, r=1 to 50, s=0 to 50, t=0 to 25, Ar= ##STR16## R2 =--(CH2 --CH═
CH--CH2)n --, --R6 --, --R6 --O--CO--R7 --CO--O--R6 -- or ##STR17## where n=1 to 50, u=0 to 150,R6 has the same meaning as R5, except that ##STR18## are excluded, and R7 has the following meaning;
##STR19## with q=2 to 4, s=0 to 50, t=0 to 25,v=0 to 20, and Ar has the meaning indicated above. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A biosensor with a selective detection system comprising a polymer and a biochemical substance wherein the detection system is produced in the following manner:
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applying an olefinic-unsaturated, epoxyfunctional polyether to a carrier material in the form of a layer, cross-linking the polyether by means of high-energy radiation to form an epoxyfunctional polymer matrix, treating the layer with an aqueous solution of the biochemical substance, whereby the biochemical substance is immobilized in the polymer matrix by reaction with epoxy groups, and stabilizing the layer by reaction of non-reacted epoxy groups with a compound containing an amino group, a carboxyl group or an amino group and a carboxyl group, wherein the polyether has the following structure;
space="preserve" listing-type="equation">R.sup.1 --O--R.sup.2 --O--R.sup.1, where R.sup.1 = ##STR20## where the following applies;
##STR21## where R.sup.3 =--(CH.sub.2).sub.m --, with m=1 to 10R4 =H or CH3 ; R2 =--(CH2 --CH═
CH--CH2)n --, --R5 --, R5 --O--CO--R6 --CO--O--R5 -- or ##STR22## where n=1 to 50, u=0 to 150,R5 = ##STR23## with p=2 to 20, q=2 to 4, r=1 to 50, s=0 to 50, t=0 to 25, Ar= ##STR24## and R6 has the following meaning;
##STR25## with q=2 to 4, s=0 to 50, t=0 to 25,v=0 to 20, and Ar has the meaning indicated above. - View Dependent Claims (8, 9, 10, 11, 12)
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Specification