Method and apparatus for detecting cataractogenesis

• US 5,392,776 A
• Filed: 09/21/1992
• Issued: 02/28/1995
• Est. Priority Date: 09/21/1992
• Status: Expired due to Fees

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Oculon Corp (W World Corp.)
• Inventors: Hayden, Douglas L., Thurston, George M., Benedek, George B., Peetermans, Joyce A., Taratuta, Victor G.
• Primary Examiner(s): Sykes, Angela D.
• Assistant Examiner(s): Nasser, Robert L.
• Application Number:
  US07/948,273
• Time in Patent Office: 890 Days
• Field of Search: 128/633, 128/664, 128/665, 128/666, 128/715, 351/205, 351/213, 351/201
• US Class Current: 600/318
• CPC Class Codes: A61B 3/10 Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
• CPC Class Codes: A61B 3/1173 for examining the eye lens

Abstract

A method and apparatus for detecting cataractogenesis is disclosed. Quasielastic light scattering data are collected from the lens of an individual to be tested for cataractogenesis. The data are collected from specific and reproducible sites within the lens by means of measurements made using a reticle in the apparatus and processed by an autocorrelator. The data from the autocorrelator are then fit to a double exponential form of autocorrelation function and the resulting functional form is transformed to produce at least one dimensionless parameter F_mos. This parameter has been found to change predictably with the individual'"'"'s age and, accordingly, is useful in detecting and determining the degree of cataractogenesis in the individual.

Claims

1. A method for in vivo detection of cataractogenesis in ocular tissue, comprising the steps of:

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the ocular tissue;

(c) collecting light that is scattered from the ocular tissue;

(d) performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including a dimensionless parameter F_mos and a diffusion decay time parameter (τ

_S), where F_mos is a measure of the proportion of an intensity of the light scattered by particles in the ocular tissue that move slowly relative to a diffusion speed of other particles in the ocular tissue and τ

_S is a diffusion decay time of the particles that move slowly relative to the diffusion speed of the other particles in the ocular tissue; and

(e) detecting cataractogenesis from the signature.

2. The method of claim 1 wherein step (c) comprises collecting the light that is scattered from the ocular tissue over a time period and step (d) comprises computing a temporal autocorrelation function of the collected light.

3. The method of claim 2 wherein step (d) further comprises performing a least squares analysis on the temporal autocorrelation function to determine the functional form of the function C(τ

) which best fits the temporal autocorrelation function, where

space="preserve" listing-type="equation">C(τ

)=A ]F.sub.mos exp(-τ

/τ

.sub.S)+(1-F.sub.mos)exp(-τ

/τ

.sub.f ].sup.2 +B,

r is a time delay variable, A is a constant representative of scattering in the absence of particles in the ocular tissue that move very slowly relative to the diffusion speed of the other particles in the ocular tissue and B is a baseline.

4. The method of claim 3, further comprising the step of:

(f) determining the value of A from in vitro experimentation in the absence of particles in the ocular tissue that move very slowly relative to the diffusion speed of the other particles in the ocular tissue.

5. The method of claim 4 wherein step (f) comprises making correlation measurements on a solution of polystyrene spheres.

6. The method of claim 4 wherein step (f) comprises making correlation measurements on a solution of material isolated from an ocular lens containing substantially no particles that move very slowly relative to the diffusion speed of the other particles in the tissue.

7. The method of claim 3, further comprising the step of:

(f) making measurements of the value of a parameter representative of light scattering from the ocular lens of each member of a population of individuals and choosing a value of A to be the maximum of the measured values.

8. Apparatus for in vivo detection of cataractogenesis in ocular tissue, comprising:

a light source producing substantially monochromatic, coherent, collimated light;

optics directing the light to impinge on the ocular tissue;

a light collector collecting light that is scattered from the ocular tissue;

correlation means for performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including a dimensionless parameter F_mos and a diffusion decay time parameter (τ

_S), where F_mos is a measure of the proportion of an intensity of the light scattered by particles in the ocular tissue that move slowly relative to a diffusion speed of other particles in the ocular tissue and τ

_S is a diffusion decay time of the particles that move slowly relative to the diffusion speed of the other particles in the ocular tissue; and

analysis means for detecting cataractogenesis from the signature.

9. The apparatus of claim 8 wherein the light collector collects light that is scattered from the ocular tissue over a predetermined period of time, said correlation means comprising a computer programmed to compute a temporal autocorrelation function of the intensity of the collected light.

10. The apparatus of claim 9 wherein said correlation means comprises means for performing a least squares analysis on the temporal autocorrelation function to determine the functional form of the function C(τ

) which best fits the temporal autocorrelation function, where

space="preserve" listing-type="equation">C(τ

)=A ]F.sub.mos exp(-τ

/τ

.sub.S)+(1-F.sub.mos)exp(-τ

/τ

.sub.f ].sup.2 +B,

τ

is a time delay variable, A is a predetermined constant representative of scattering in the absence of particles in the ocular tissue that move very slowly relative to the diffusion speed of the other particles in the ocular tissue and B is a baseline.

11. The apparatus of claim 10, further comprising means for determining the value of A from in vitro experimentation in the absence of particles in the ocular tissue that move very slowly relative to the diffusion speed of the other particles in the ocular tissue.

12. The apparatus of claim 11 wherein said means for determining the value of A comprises means for making correlation measurements on a solution of polystyrene spheres.

13. The apparatus of claim 11 wherein said means determining the value of A comprises means for making correlation measurements on a solution of material isolated from an ocular lens containing substantially no particles that move very slowly relative to other particles in the tissue.

14. The apparatus of claim 10 further comprising parameter measurement means for making measurements of the value of a parameter representative of light scattering from the ocular lens of each member of a population of individuals and choosing a value of A to be the maximum of the measured values.

15. The apparatus of claim 8 wherein said correlation means comprises an integrated circuit for computing a temporal autocorrelation function of the intensity of the collected light.

16. The apparatus of claim 8 wherein said correlation means comprises a standalone autocorrelator for computing a temporal autocorrelation function of the intensity of the collected light.

17. A method for in vivo detection of cataractogenesis in ocular tissue of an individual subject, comprising the steps of:

(1) For each subject in a population of subjects;

(a) producing substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the subject'"'"'s ocular tissue;

(c) collecting light that is scattered from the ocular tissue; and

(d) performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including a dimensionless parameter F_mos and a diffusion decay time parameter (τ

_S), where F_mos is a measure of the proportion of an intensity of the light scattered by particles in the ocular tissue that move slowly relative to a diffusion speed of other particles in the ocular tissue and τ

_S is a diffusion decay time of the particles that move slowly relative to the diffusion speed of the other particles in the ocular tissue;

(2) developing a frequency distribution of at least one of the parameters;

(3) for the individual subject;

(a) producing substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the individual subject'"'"'s ocular tissue;

(c) collecting light that is scattered from the individual subject'"'"'s ocular tissue; and

(d) performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including a dimensionless parameter F_mos and a diffusion decay time parameter (τ

_S), where F_mos is a measure of the proportion of an intensity of the light scattered by particles in the ocular tissue that move slowly relative to a diffusion speed of other particles in the ocular tissue and τ

_S is a diffusion decay time of the particles that move slowly relative to the diffusion speed of the other particles in the ocular tissue; and

(4) determining a rate of cataractogenesis for the individual subject, based on the position of a value of at least one variable for the individual subject relative to the frequency distribution for that variable.

18. The method of claim 17, wherein step (2) comprises developing a frequency distribution of exactly one of the parameters taken from the group of parameters comprising F_mos and τ

_S.

19. The ;

method of claim 18, wherein step (2) comprises developing a frequency distribution of exactly two of the parameters taken from the group of parameters consisting of F_mos and τ

_S.

20. The method of claim 17, wherein step (2) comprises developing a frequency distribution of exactly one of the parameters.

21. Apparatus for in vivo detection of cataractogenesis in ocular tissue of an individual subject, comprising:

means for analyzing subjects, including, for each subject;

a light source producing substantially monochromatic, coherent, collimated light;

optics directing the light to impinge on the subject'"'"'s ocular tissue;

a light collector collecting light that is scattered from the subject'"'"'s ocular tissue; and

analysis means for performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including a dimensionless parameter F_mos and a diffusion decay time parameter (τ

_S), where F_mos is a measure of the proportion of an intensity of the light scattered by particles in the ocular tissue that move slowly relative to a diffusion speed of other particles in the ocular tissue and τ

_S is a diffusion decay time of the particles that move slowly relative to the diffusion speed of the other particles in the ocular tissue;

means for developing a frequency distribution of at least one of the parameters; and

means for determining a rate of cataractogenesis for an individual subject, by comparing the value of at least one parameter of the individual subject to the frequency distribution for the parameter.

22. The apparatus of claim 21, wherein said means for developing a frequency distribution develops a frequency distribution of exactly one of the parameters taken from the group of parameters comprising F_mos and τ

_S.

23. The apparatus of claim 22, wherein said means for developing a frequency distribution develops a frequency distribution of exactly two of the parameters taken from the group of parameters consisting of F_mos and τ

_S.

24. The apparatus of claim 21, wherein said means for developing a frequency distribution develops a frequency distribution of exactly one of the parameters.

25. A method for in vivo detection of cataractogenesis in ocular tissue of an individual subject, comprising the steps of:

(1) For the individual subject, at a first time;

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the ocular tissue of the individual subject;

(c) collecting light that is scattered from the ocular tissue of the individual subject; and

(d) performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including a dimensionless parameter F_mos and a diffusion decay time parameter (τ

_S), where F_mos is a measure of the proportion of an intensity of the light scattered by particles in the ocular tissue that move slowly relative to a diffusion speed of other particles in the ocular tissue and τ

_S is a diffusion decay time of the particles that move slowly relative to the diffusion speed of the other particles in the ocular tissue;

(2) for the individual subject, at a second time following the first time;

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the ocular tissue of the individual subject;

(c) collecting light that is scattered from the ocular tissue of the individual subject; and

(d) performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including a dimensionless parameter F_mos and a diffusion decay time parameter (τ

_S), where F_mos is a measure of the proportion of an intensity of the light scattered by particles in the ocular tissue that move slowly relative to a diffusion speed of other particles in the ocular tissue and τ

_S is a diffusion decay time of the particles that move slowly relative to the diffusion speed of the other particles in the ocular tissue;

(3) determining a rate of change of at least one of the variables of the individual subject'"'"'s ocular tissue as a function of age by comparing the at least one variable determined at said first time with the at least one variable determined at said second time;

(4) for each individual in a population of individuals, at a third time;

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the ocular tissue of each individual subject;

(c) collecting light that is scattered from the ocular tissue of each individual subject; and

(d) performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including a dimensionless parameter F_mos and a diffusion decay time parameter (τ

_S), where F_mos is a measure of the proportion of an intensity of the light scattered by particles in the ocular tissue that move slowly relative to a diffusion speed of other particles in the ocular tissue and τ

_S is a diffusion decay time of the particles that move slowly relative to the diffusion speed of the other particles in the ocular tissue; and

(5) comparing the rate of change with individual age of the at least one variable for the particular individual subject to a normal value of the rate of change with age of the at least one variable for the population of subjects by comparing the rate of change of the at least one variable determined at said second time with the at least one variable determined at said third time to detect cataractogenesis in the ocular tissue of the individual subject.

26. Apparatus for in vivo detection of cataractogenesis in ocular tissue of an individual subject, comprising:

means for analyzing the ocular tissue of the individual subject, at a first time, for analyzing the ocular tissue of the individual subject at a second time following said first time, and for analyzing the ocular tissue of each individual in a population of individuals at a third time, including;

a light source for producing substantially monochromatic, coherent, collimated light;

optics for directing the light to impinge on the ocular tissue of the individual subject and the individual in the population of individuals;

a light collector for collecting light that is scattered from the ocular tissue of the individual subject and the individual in the population of individuals;

electrical circuitry for performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including a dimensionless parameter F_mos and a diffusion decay time parameter (τ

_S), where F_mos is a measure of the proportion of an intensity of the light scattered by particles in the ocular tissue that move slowly relative to a diffusion speed of other particles in the ocular tissue and τ

_S is a diffusion decay time of the particles that move slowly relative to the diffusion speed of the other particles in the ocular tissue;

means for determining a rate of change of at least one of the variables of the individual subject'"'"'s ocular tissue as a function of age; and

means for comparing the rate of change of the at least one variable for the particular individual subject to a normal value of the rate of change with age of the at least one variable for the population of subjects to detect cataractogenesis in the ocular tissue of the individual subject.

27. A method for in vivo detection of cataractogenesis in ocular tissue, comprising the steps of:

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the ocular tissue;

(c) collecting light that is scattered from the ocular tissue for a predetermined period of time, the scattered light having a fluctuating intensity;

(d) performing a mathematical analysis on the intensity and the fluctuations of the intensity of the collected light, thereby determining a signature of cataractogenesis, the signature including an intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to said predetermined period of time; and

(e) determining from the signature the degree of cataractogenesis.

28. The method of claim 27 wherein step (d) comprises computing a temporal autocorrelation function of the fluctuations of the intensity of the collected light.

29. Apparatus for in vivo detection of cataractogenesis in ocular tissue, comprising:

a light source producing a substantially monochromatic, coherent, collimated light;

optics directing the light to impinge on the ocular tissue;

a light collector for collecting light that is scattered from the ocular tissue for a predetermined period of time, the scattered light having a fluctuating intensity;

analysis means for performing a mathematical analysis on the intensity and the fluctuations of the intensity of the collected light, thereby determining a signature of cataractogenesis, the signature including an intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to said predetermined period of time; and

means for determining from the signature the degree of cataractogenesis.

30. The apparatus of claim 29 wherein said analysis means computes a temporal autocorrelation function of the fluctuations of the intensity of the collected light.

31. A method for in vivo detection of cataractogenesis in ocular tissue, comprising the steps of:

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the ocular tissue;

(c) collecting light that is scattered from the ocular tissue;

(d) performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including an intensity (I_mof) of the light scattered by fast moving particles, a diffusion decay time (τ

_f) of fast moving particles, an intensity (I_mos) of the light scattered by slow moving particles, a diffusion decay time (τ

_S) of slow moving particles, and an intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to a diffusion speed of other particles in the ocular tissue; and

(e) determining from the signature the degree of cataractogenesis.

32. The method of claim 31 wherein step (d) comprises computing a temporal autocorrelation function of the collected light.

33. The method of claim 32 wherein step (d) further comprises performing a least squares analysis on the temporal autocorrelation function to determine the functional form of the function C(τ

) which best fits the temporal autocorrelation function, where

space="preserve" listing-type="equation">C(τ

)=α

.sub.0 [I.sub.mof exp(-τ

/τ

.sub.f)+I.sub.mos exp(-τ

/τ

.sub.S)].sup.2 +[I.sub.mof +I.sub.mos +I.sub.movs ].sup.2,

τ

is a time delay variable, and α

₀ is a predetermined constant representative of scattering in the absence of particles in the ocular tissue that move very slowly relative to the diffusion speed of other particles in the ocular tissue.

34. The method of claim 33, further comprising the step of:

(f) determining the value of α

₀ from in vitro experimentation in the absence of particles in the ocular tissue that move very slowly relative to the diffusion speed of other particles in the ocular tissue.

35. The method of claim 34 wherein step (f) comprises making correlation measurements on a solution of polystyrene spheres.

36. The method of claim 34 wherein step (f) comprises making correlation measurements on a solution of material isolated from an ocular lens containing substantially no particles that move very slowly relative to the diffusion speed of other particles in the tissue.

37. The method of claim 33, further comprising the step of:

(f) making measurements of the value of a parameter representative of light scattering from the ocular lens of each member of a population of individuals and choosing a value of α

₀ to be the maximum of the measured values.

38. Apparatus for in vivo detection of cataractogenesis in ocular tissue, comprising:

a light source producing substantially monochromatic, coherent, collimated light;

optics directing the light to impinge on the ocular tissue;

a light collector collecting light that is scattered from the ocular tissue;

analysis means for performing a correlation analysis on the collected light to determine a signature of cataractogenesis, the signature including an intensity (I_mof) of the light scattered by fast moving particles, a diffusion decay time (τ

_f) of fast moving particles, an intensity (I_mos) of the light scattered by slow moving particles, a diffusion decay time (τ

_S) of slow moving particles, and an intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to a diffusion speed of the other particles in the ocular tissue; and

means for determining from the signature the degree of cataractogenesis.

39. The apparatus of claim 38 wherein said analysis means comprises a computer programmed to compute a temporal autocorrelation function of the intensity of the collected light.

40. The apparatus of claim 38 wherein said analysis means comprises an integrated circuit for computing a temporal autocorrelation function of the intensity of the collected light.

41. The apparatus of claim 38 wherein said analysis means comprises a standalone autocorrelator for computing a temporal autocorrelation function of the intensity of the collected light.

42. The apparatus of claim 38 wherein said analysis means comprises means for performing a least squares analysis on the temporal autocorrelation function to determine the functional form of the function C(τ

) which best fits the temporal autocorrelation function, where

space="preserve" listing-type="equation">C(τ

)=α

.sub.0 [I.sub.mof exp(-τ

/τ

.sub.f)+I.sub.mos exp(-τ

/τ

.sub.S)].sup.2 +[I.sub.mof +I.sub.mos +I.sub.movs ].sup.2,

τ

is a time delay variable, and α

₀ is a predetermined constant representative of scattering in the absence of particles in the ocular tissue that move very slowly relative to the diffusion speed of other particles in the ocular tissue.

43. The apparatus of claim 42, further comprising means for determining the value of α

₀ from in vitro experimentation in the absence of particles in the ocular tissue that move very slowly relative to the diffusion speed of the other particles in the ocular tissue.

44. The apparatus of claim 43 wherein the means for determining the value of α

₀ comprises means for making correlation measurements on a solution of polystyrene spheres.

45. The apparatus of claim 43 wherein the means for determining the value of α

₀ comprises means for making correlation measurements on a solution of material isolated from an ocular lens containing substantially no particles that move very slowly relative to the diffusion speed of other particles in the tissue.

46. The apparatus of claim 42 wherein said analysis means includes means for making measurements of the value of a parameter representative of light scattering from the ocular lens of each member of a population of individuals and for choosing a value of α

₀ to be the maximum of the measured values.

47. A method for in vivo detection of cataractogenesis in ocular tissue of an individual subject, comprising the steps of:

(1) For each subject in a population of subjects;

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on subject'"'"'s ocular tissue;

(c) collecting light that is scattered from the ocular tissue; and

(d) performing a correlation analysis on the collected light to determine values of variables which include an intensity (I_mos) of the light scattered by slow moving particles, a diffusion decay time (τ

_S) of the slow moving particles, and an intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to a diffusion speed of the other particles in the ocular tissue;

(2) developing a frequency distribution of at least one of the variables;

(3) for the individual subject;

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the individual subject'"'"'s ocular tissue;

(c) collecting light that is scattered from the individual subject'"'"'s ocular tissue; and

(d) performing a correlation analysis on the collected light to determine values of variables which include an intensity (I_mos) of the light scattered by slow moving particles, a diffusion decay time (τ

_S) of the slow moving particles, and an intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to a diffusion speed of the other particles in the ocular tissue; and

(4) determining a rate of cataractogenesis for the individual subject, based on the position of the value of at least one variable for the individual subject relative to the frequency distribution for that variable.

48. The method of claim 47, wherein step (2) comprises developing a frequency distribution of two or more of the variables.

49. The method of claim 48, wherein step (2) comprises developing a frequency distribution of exactly two of the variables taken from the group of variables consisting of I_mos, τ

_S, and I_movs.

50. The method of claim 47, wherein step (2) comprises developing a frequency distribution of exactly one of the variables.

51. Apparatus for in vivo detection of cataractogenesis in ocular tissue of an individual subject, comprising:

means for analyzing the individual subject and subjects in a population, including, for each subject;

a light source producing substantially monochromatic, coherent, collimated light;

optics directing the light to impinge on the subject'"'"'s ocular tissue;

a light collector collecting light that is scattered from the subject'"'"'s ocular tissue;

analysis means for performing a correlation analysis on the collected light to determine values of variables, including an intensity (I_mos) of the light scattered by slow moving particles, a diffusion decay time (τ

_S) of the slow moving particles in the subject'"'"'s ocular tissue, and an intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to a diffusion speed of the other particles in the ocular tissue;

means for developing a frequency distribution of at least one of the variables and from the values of variables for the population of subjects; and

means for determining a rage of cataractogenesis for the individual subject by comparing the value of at least one variable for the individual subject to the frequency distribution for the variable.

52. The apparatus of claim 51, wherein said means for developing a frequency distribution develops a frequency distribution of two or more of the variables.

53. The apparatus of claim 52, wherein said means for developing a frequency distribution develops a frequency distribution of exactly two of the variables taken from the group of variables consisting of I_mos, τ

_S, and I_movs.

54. The apparatus of claim 51, wherein said means for developing a frequency distribution develops a frequency distribution of exactly one of the variables.

55. The method for in vivo detection of cataractogenesis in ocular tissue of an individual subject, comprising the steps of:

(1) For the individual subject, at a first time;

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the ocular tissue of the individual subject;

(c) collecting light that is scattered from the ocular tissue of the individual subject over a predetermined first time period; and

(d) performing a correlation analysis on the collected light to determine values of variables, including an intensity (I_mos) of the light scattered by slow moving particles in the individual subject'"'"'s ocular tissue, a diffusion decay time (τ

_S) of the slow moving particles in the individual subject'"'"'s ocular tissue, and an intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to the first time period over which the light is collected in the individual subject'"'"'s ocular tissue;

(2) for the individual subject, at a second time following the first time;

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the ocular tissue of the individual subject;

(c) collecting light that is scattered form the ocular tissue of the individual subject over a predetermined second time period; and

(d) performing a correlation analysis on the collected light to determine values of variables, including an intensity (I_mos) of the light scattered by slow moving particles in the individual subject'"'"'s ocular tissue, a diffusion decay time (τ

_S) of the slow moving particles in the individual subject'"'"'s ocular tissue, and an intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to the second time period over which the light is collected in the ocular tissue;

(3) determining a rate of change of at least one of the variables of the individual subject'"'"'s ocular tissue as a function of age;

(4) for each individual in a population of individuals, at a third time;

(a) producing a substantially monochromatic, coherent, collimated light;

(b) causing the light to impinge on the ocular tissue of each individual in the population of individuals;

(c) collecting light that is scattered from the ocular tissue of each individual in the population of individuals over a predetermined third time period; and

(d) performing a correlation analysis on the collected light to determine normal values of the rate of change with individual age of the variables for the population, including an intensity (I_mos) of the light scattered by slow moving particles within the ocular tissue of individuals in the population of individuals, a diffusion decay time (τ

_S) of the slow moving particles in the subject'"'"'s ocular tissue, and an intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to the their time period over which the light is collected in the ocular tissue; and

(5) comparing the rate of change with individual age of the at least one variable for the particular individual subject to the normal value of the rate of change with age of the at least one variable for the population of subjects to detect cataractogenesis in the ocular tissue of the individual subject.

56. Apparatus for in vivo detection of cataractogenesis in ocular tissue of an individual subject, comprising:

means for analyzing the ocular tissue of the individual subject at a first time, for analyzing the ocular tissue of the individual subject at a second time following the first time and for analyzing the ocular tissue of each individual in a population of individuals at a third time including;

a light source for producing substantially monochromatic, coherent, collimated light;

optics for directing the light to impinge on the ocular tissue of the individual subject and individuals in the population;

a light collector for collecting light that is scattered from the ocular tissue of the individual subject over a predetermined first time period; and

electrical circuitry for performing a correlation analysis on the collected light to determine the values of variables, including the intensity (I_mos) of the light scattered by slow moving particles, the diffusion decay time (τ

_S) of the slow moving particles, and the intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to the first time period over which the light is collected in the ocular tissue of the subject;

means for analyzing the ocular tissue of the individual subject at a second time following the first time, including;

a light source for producing substantially monochromatic, coherent, collimated light;

optics for directing the light to impinge on the ocular tissue of the individual subject;

a light collector for collecting light that is scattered from the ocular tissue of the individual subject over a predetermined second time period; and

electrical circuitry for performing a correlation analysis on the collected light to determine the values of variables, including the intensity (I_mos) of the light scattered by slow moving particles, the diffusion decay time (τ

_S) of the slow moving particles, and the intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to the second time period over which the light is collected in the ocular tissue of the subject;

apparatus for determining the rate of change of at least one of the variables o the individual subject'"'"'s ocular tissue as a function of age;

means for analyzing the ocular tissue of each individual in a population of individuals at a third time, including;

a light source for producing substantially monochromatic, coherent, collimated light;

optics for directing the light to impinge on the ocular tissue of each individual subject;

a light collector for collecting light that is scattered from the ocular tissue of each individual subject over a predetermined third time period; and

electrical circuitry for performing a correlation analysis on the collected light to determine normal values of the variables for the population, including the intensity (I_mos) of the light scattered by slow moving particles, the diffusion decay time (τ

_S) of the slow moving particles, and the intensity (I_movs) of the light scattered by particles in the ocular tissue that move very slowly relative to the third time period over which the light is collected in the ocular tissue of the subject; and

electrical circuitry comparing the rate of change of the at least one variable for the particular individual subject to the normal value of the rate of change with age of the at least one variable for the population of subjects to detect cataractogenesis in the ocular tissue of the individual subject.