Zero volume electrochemical cell

• US 5,395,503 A
• Filed: 09/29/1993
• Issued: 03/07/1995
• Est. Priority Date: 06/20/1986
• Status: Expired due to Fees

• Current Assignee: Molecular Devices
• Original Assignee: Molecular Devices
• Inventors: Zuk, Robert F., Parce, John W.
• Primary Examiner(s): TUNG, TA HSUNG
• Application Number:
  US08/129,541
• Time in Patent Office: 524 Days
• Field of Search: 204/153.1, 204/153.12, 204/400, 204/403, 204/409, 204/415, 204/416, 204/418, 204/419, 204/225, 204/222
• US Class Current: 204/403.1
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements

Abstract

Methods and apparatuses are described for determining the presence of an analyte in a sample suspected of containing the analyte. The method comprises contacting a concentrate of a determinable element capable of being detected by means of a semiconductive electrode with the electrode in the presence of a relatively large volume of an assay medium. The determinable element is present in the concentrate in the amount related to the amount of analyte present in the sample. The volume of the medium in diffusive communication with the concentrate is then reduced and the determinable element is detected by means of the effect that the determinable element has on the semiconductive electrode.

Claims

1. An apparatus for detecting an analyte comprising a porous membrane with an enzyme affixed thereto, the apparatus comprising:

an assay chamber adapted to removably receive the porous membrane;

a working electrode and a plunger means as confronting walls of the assay chamber;

means for moving the plunger means with respect to the working electrode from a distal position to a proximal confronting position so as to confine the porous membrane within 1 millimeter of the working electrode; and

means for connecting the working electrode to an external circuit for measuring changes in an electrical characteristic of the working electrode in response to the enzyme on the porous membrane.

2. An apparatus as in claim 1 wherein, in the proximal confronting position, the plunger means is not more than 100 microns from the working electrode.

3. An apparatus as in claim 1, wherein in the proximal confronting position, the plunger means is within 14-400 microns from the working electrode.