Control of flow cytometer having vacuum fluidics
First Claim
1. A flow cytometer control system for controlling the delivery of a cell sample drawn from a vessel, comprising:
- a flow cell, in communication with a cell sample vessel, for performing cell sample analysis;
an intake passage, connected to said flow cell, providing an ingress communication path for sheath fluid into said flow cell;
first outlet passage, connected to said flow cell, providing a first egress communication path for said sheath fluid out of said flow cell;
a vacuum pump with motor means, in vacuum communication with said first outlet passage and a second outlet passage, for pulling said sheath fluid through said flow cell;
flow resistor means, connected to said intake passage, for developing a pressure drop along said intake passage to cause cell sample to be aspirated from said vessel into said flow cell;
a first valve, connected to said intake passage, for controlling the flow of said sheath fluid into said flow cell at a predetermined cell sample flow rate;
a second valve, connected to said first outlet passage, for controlling the flow of said sheath fluid out of said flow cell;
control means for opening said second valve for a predetermined time prior to opening said first valve in order to permit a vacuum to be developed in said flow cell before opening said first valve thereby pulling cell sample through said flow cell to increase sample flow into said analysis region; and
said control means being operatively connected to said first and second valves.
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Accused Products
Abstract
The present invention relates to a system for controlling the movement or transport of objects such as cells and other particles in a moving liquid stream. More particularly the invention relates to a system including a vacuum pump which pulls the sheath fluid from an open supply reservoir through a flow cell assembly where cell analysis occurs and discharges the flow cell effluent to an open waste reservoir. A pressure drop is created through the conduit leading from the supply reservoir to the flow cell which aspirates the cell suspension from an open sample vessel into and through the flow cell. The vacuum is regulated by controlling the electric power applied to the vacuum pump motor. Starting, stopping, reverse flushing, and draining of the flow cell assembly are controlled by programming the operation of four electric solenoid valves in fluid communication with the flow cell inlet and outlet passages, the operation of a reverse flush pump and, the operation of an electrically controlled tube lifter. A deaerator is provided to remove some of the air dissolved in the sheath fluid provided to the flow cell assembly to prevent the release of air and formation of adherent air bubbles in sensitive areas of the flow cell assembly.
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Citations
11 Claims
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1. A flow cytometer control system for controlling the delivery of a cell sample drawn from a vessel, comprising:
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a flow cell, in communication with a cell sample vessel, for performing cell sample analysis; an intake passage, connected to said flow cell, providing an ingress communication path for sheath fluid into said flow cell; first outlet passage, connected to said flow cell, providing a first egress communication path for said sheath fluid out of said flow cell; a vacuum pump with motor means, in vacuum communication with said first outlet passage and a second outlet passage, for pulling said sheath fluid through said flow cell; flow resistor means, connected to said intake passage, for developing a pressure drop along said intake passage to cause cell sample to be aspirated from said vessel into said flow cell; a first valve, connected to said intake passage, for controlling the flow of said sheath fluid into said flow cell at a predetermined cell sample flow rate; a second valve, connected to said first outlet passage, for controlling the flow of said sheath fluid out of said flow cell; control means for opening said second valve for a predetermined time prior to opening said first valve in order to permit a vacuum to be developed in said flow cell before opening said first valve thereby pulling cell sample through said flow cell to increase sample flow into said analysis region; and said control means being operatively connected to said first and second valves. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification