Method for diagnosing rheumatoid arthritis
First Claim
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1. A method for diagnosing rheumatoid arthritis comprising the steps of:
- (a) providing a recombinant RAMA antigen that is specifically detectable by rheumatoid arthritis-associated IgM antibodies;
(b) incubating said RAMA antigen with patient serum to form a complex of said RAMA antigen and said IgM antibodies; and
(c) detecting said complex as a measure of said IgM antibodies against said RAMA antigen in patient sera.
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Abstract
A method is described for diagnosing rheumatoid arthritis by providing a recombinant IgM-specific rheumatoid arthritis-associated antigen and detecting antibodies against the antigen in patient sera. Preliminary steps of making a cDNA library from polyadenylated RNA purified from human cells, selecting recombinants that express the antigen, recloning cDNA containing the antigen gene in a high level expression vector, expressing the antigen in transformed cells, and purifying the antigen are also described.
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Citations
23 Claims
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1. A method for diagnosing rheumatoid arthritis comprising the steps of:
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(a) providing a recombinant RAMA antigen that is specifically detectable by rheumatoid arthritis-associated IgM antibodies; (b) incubating said RAMA antigen with patient serum to form a complex of said RAMA antigen and said IgM antibodies; and (c) detecting said complex as a measure of said IgM antibodies against said RAMA antigen in patient sera. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification