Acrylic polymer electrophoresis support media

• US 5,397,449 A
• Filed: 04/06/1994
• Issued: 03/14/1995
• Est. Priority Date: 07/14/1992
• Status: Expired due to Fees

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Zewert, Thomas, Harrington, Michael G.
• Primary Examiner(s): Schofer, Joseph L.
• Assistant Examiner(s): SAROFIM, NABIL F
• Application Number:
  US08/223,582
• Time in Patent Office: 342 Days
• Field of Search: 204/182.8, 204/299 R, 526/320, 526/934, 436/516
• US Class Current: 204/469
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture
• CPC Class Codes: Y10S 526/934 Electrodeposit, e.g. electrophoretic, xerographic

Abstract

Electrophoresis support media which include one or more polymers made from hydroxy alkyl esters of acrylic or methylacrylic acid or poly (alkylene glycol) esters of acrylic or methacrylic acid. These acrylic or methacrylic monomers are polymerized or copolymerized to form electrophoresis support media which can be used in place of polyacrylamide gels, agar gels and agarose gels. The acrylic or methacrylic ester monomers may be cross-linked with various cross-linking agents to provide electrophoresis support media with a wide range of pore sizes and physical strength. The electrophoresis support media is well-suited for use with organic solvents.

Claims

1. An electrophoresis support media comprising a polymer gel which is made from a monomer selected from the group consisting of hydroxyalkyl esters of acrylic or methacrylic acid, said polymer gel being in the shape of a slab or column, said polymer gel slab or column comprising at least one well into which samples are loaded for electrophoresis.

2. An electrophoresis support media according to claim 1 which further includes a cross-linking agent selected from the group consisting of tetraethyleneglycol acrylate, tetraethyleneglycol methacrylate, pentaerythritol di(methacrylate), poly(ethyleneglycol) diacrylate, polyethyleneglycol dimethacrylate, bisacrylamide and piperazine diacrylate.

3. An electrophoresis support media according to claim 1 wherein the molecular weight of said polymer is from between 100,000 to 5,000,000.

4. An electrophoresis support media according to claim 1 wherein said media consists essentially of a single polymer.

5. An electrophoresis support media according to claim 4 wherein said monomer from which said polymer is made is glycerol acrylate or methacrylate.

6. An electrophoresis support media according to claim 4 wherein said monomer from which said polymer is made is a hydroxyalkyl acrylate or methacrylate.

7. An electrophoresis support media according to claim 1 wherein said media further comprises acrylamide monomer copolymerized with said monomer to form a copolymer support media wherein the weight percent of said acrylamide in said copolymer support media is less than 70 weight percent.

8. An electrophoresis system comprising a support media according to claim 1 and an electrophoresis solvent dispersed within said support media.

9. An electrophoresis system according to claim 8 wherein said electrophoresis solvent comprises an organic solvent.