Infant formula and baby food containing docosahexaenoic acid obtained from dinoflagellates

• US 5,397,591 A
• Filed: 08/13/1992
• Issued: 03/14/1995
• Est. Priority Date: 02/13/1990
• Status: Expired due to Term

• Current Assignee: Martek Biosciences Corporation (DSM-Firmenich AG)
• Original Assignee: Martek Biosciences Corporation (DSM-Firmenich AG)
• Inventors: Kyle, David J., Sicotte, Valerie J., Reeb, Sue E.
• Primary Examiner(s): Robinson, Douglas W.
• Assistant Examiner(s): Weber, Jon P.
• Application Number:
  US07/916,874
• Time in Patent Office: 943 Days
• Field of Search: 426/417, 426/442, 426/601, 426/801, 426/648, 426/602, 435/134, 435/257.1, 435/946
• US Class Current: 426/602
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 50/80 for aquatic animals, e.g. fish, crustaceans or molluscs
• CPC Class Codes: A23L 33/115 Fatty acids or derivatives thereof; Fats or oils
• CPC Class Codes: A61P 3/00 Drugs for disorders of the metabolism of the blood or the extracellular fluid A61P7/00
• CPC Class Codes: A61P 3/02 Nutrients, e.g. vitamins, minerals
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: C11B 1/10 by extracting
• CPC Class Codes: C12N 1/125 Unicellular algae isolates
• CPC Class Codes: C12P 7/6472 containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
• CPC Class Codes: C12R 2001/89 Algae ; Processes using algae
• CPC Class Codes: Y10S 426/801 Pediatric
• CPC Class Codes: Y10S 426/805 Pet food for dog, cat, bird, or fish
• CPC Class Codes: Y10S 435/946 using algae

Abstract

Infant formula and baby food compositions are presented which contain single cell edible oil which is recovered from dinoflagellates and which lacks unpleasant tastes and fishy odors. This single cell edible oil comprises at least 70% triglycerides which contain about 20-35% docosahexaenoic acid (DHA) and lack eicosapentaenoic acid (EPA). To produce the single cell oil, the dinoflagellates are cultivated in fermentors and induced to produce the single cell oil which is subsequently recovered by extraction with solvents.

Claims

1. An infant formula comprising a single cell edible oil recovered from a dinoflagellate, which oil comprises at least 70% triglycerides containing at least about 20% DHA, said oil containing no significant quantity of EPA.

2. The infant formula of claim 1, wherein said oil comprises about 0.05% by weight (50 mg/100 ml) of said formula.

3. The infant formula of claim 2, wherein said oil comprises at least about 30% DHA.

4. Infant formula according to claim 1, comprising a single cell oil which comprises at least 35% DHA, and no significant quantity of EPA.

5. An infant formula in accordance with claim 1, wherein the dinoflagellate is a Crypthecodinium sp.

6. Infant formula comprising a single cell edible oil which comprises at least about 70% triglycerides containing at least about 20% DHA which is produced by a process comprising:

cultivating heterotrophic microalgae of the class Dinophyceae in an aerated fermentor containing a nutrient solution having a limiting nitrogen source and an oxygen level of at least about 10% of air saturation level and continuing cultivation to achieve a cell density of at least about 10 grams biomass per liter of nutrient solution,

wherein the concentration of the nitrogen source in the nutrient solution is limited sufficiently to induce said microalgae to produce the single cell oil at a concentration of at least 1.5 grams per liter of nutrient solution, and

recovering single cell oil containing at least 70% triglycerides.

7. An infant formula in accordance with claim 6, wherein said microalgae comprise a Crypthecodinium sp.

8. An infant formula in accordance with claim 7, wherein said Crypthecodinium sp. comprises Crypthecodinium cohnii.

9. Baby food comprising a single cell edible oil recovered from a dinoflagellate, which oil comprises at least 70% triglycerides containing at least about 20% DHA, said oil containing no significant quantity of EPA.

10. The baby food of claim 9, wherein said oil comprises about 0.5% by weight of said food.

11. The baby food of claim 10, wherein said oil comprises at least about 35% DHA.

12. Baby food according to claim 9, comprising a single cell oil which contains at least 35% DHA, and no significant quantity of EPA.

13. A baby food in accordance with claim 9, wherein the dinoflagellate is a Crypthecodinium sp.

14. Baby food comprising a single cell edible oil which comprises at least about 70% triglycerides containing at least about 20% DHA which is produced by a process comprising:

cultivating heterotrophic microalgae of the class Dinophyceae in an aerated fermentor containing a nutrient solution having a limiting nitrogen source and an oxygen level of at least about 10% of air saturation level and continuing cultivation to achieve a cell density of at least about 10 grams biomass per liter of nutrient solution,

wherein the concentration of the nitrogen source in the nutrient solution is limited sufficiently to induce said microalgae to produce the single cell oil at a concentration of at least 1.5 grams per liter of nutrient solution, and

recovering single cell oil containing at least 70% triglycerides.

15. A baby food in accordance with claim 14, wherein said microalgae comprise a Crypthecodinium sp.

16. A baby food in accordance with claim 15, wherein said Crypthecodinium sp. comprises Crypthecodinium cohnii.