Methods and apparatus for automated on-column injection using a slender needle

• US 5,400,666 A
• Filed: 06/01/1994
• Issued: 03/28/1995
• Est. Priority Date: 11/04/1992
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Hewlett-Packard Company (HP Inc.)
• Inventors: Song, Wei J.
• Primary Examiner(s): RAEVIS, ROBERT R
• Application Number:
  US08/252,955
• Time in Patent Office: 300 Days
• Field of Search: 73/19.02, 73/23.41, 73/61.55, 73/61.56, 73/863.11, 73/864.21-864.25, 73/864.74, 73/864.81-864.87
• US Class Current: 73/864.21
• CPC Class Codes: G01N 2030/025 Gas chromatography
• CPC Class Codes: G01N 2030/165 retention gaps
• CPC Class Codes: G01N 30/18 using a septum or microsyringe
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums

Abstract

Methods and apparatus for preventing the failure of slender syringe needles used for on column injection in gas chromatographs are disclosed. A needle holder designed to support the needle precludes bucking deflection beyond the maximum allowable value. In a preferred embodiment, the needle used is a tapered needle that has a proximal diameter and a distal diameter that is smaller that the proximal diameter. In these embodiments, the diameter of the bore through the needle holder is slightly larger than the proximal diameter to permit the full insertion of the entire needle structure. The needle holder bore diameter is sufficiently small, however, to preclude catastrophic failure due to buckling.

Claims

1. A system for performing on-column injection of a sample into a chromatographic device, comprising:

a syringe;

a hollow needle connected to the syringe for passage of the sample therethrough, the needle having a base portion and an end portion, the end portion having an outside diameter less than that of the base portion; and

a needle support having a bore therethrough, the diameter of said bore being sufficient to enclose and thereby restrict the deflection of at least one of said base portion and end portion so enclosed to less than a predetermined maximum deflection y_m ; and

a positioning mechanism operatively connected to the needle and the needle support for effecting automated relative axial movement of the needle with respect to the needle support, said movement being between a first position wherein the end portion of the needle is substantially enclosed within the needle support, and a second position wherein the end portion is extended substantially outside the needle support.

2. The system of claim 1, wherein bore has a bore diameter that is at least 20 μ

m larger than the outside diameter of the needle.

3. The system of claim 2, wherein the outside diameter is less than 530 μ

m.

4. The system of claim 2, wherein the outside diameter is less than 300 μ

m.

5. The system of claim 2, wherein the outside diameter is between about 125 μ

m and 530 μ

m.

6. The system of claim 2, wherein the outside diameter is about 0.0091 inches (230 μ

m) and the bore diameter is about 0.0193 inches (490 μ

m).