Plastic optical biomaterials assay device

• US 5,401,469 A
• Filed: 05/06/1994
• Issued: 03/28/1995
• Est. Priority Date: 04/19/1989
• Status: Expired due to Term

• Current Assignee: Ibiden Co., Ltd.
• Original Assignee: Ibiden Co., Ltd.
• Inventors: Honda, Hiroyuki, Kobayashi, Takeshi, Shimada, Ken-ichi
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Le, Long V.
• Application Number:
  US08/238,782
• Time in Patent Office: 326 Days
• Field of Search: 422/68.1, 422/82.05-82.11, 436/164, 436/172, 436/531, 436/532, 436/535, 436/800, 436/805
• US Class Current: 422/82.07
• CPC Class Codes: G01N 33/533 with fluorescent label
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

A reagent for biomaterials assay of (fluorescent dye)_n --avidin--a compound having a plurality of reactive groups--a biomaterial, or a reagent in which avidin is bound to a compound having a plurality of reactive groups through biotin in the above, a plastic fiber optics necessary for utilizing this, an assaying device having a sensing portion utilizing this fiber optics and an assaying method of biomaterials by use of this reagent and device. The assay method can be used for an immunoassay of biomaterials such as antigens, antibodies, and enzymes contained in extremely minute amounts in blood or body fluid for medical diagnosis, and improves the detectable sensitivity to a great extent.

Claims