Histological composition for light microscopy

• US 5,401,625 A
• Filed: 06/24/1993
• Issued: 03/28/1995
• Est. Priority Date: 06/24/1993
• Status: Expired due to Term

• Current Assignee: EK Industries Incorporated
• Original Assignee: E&W Industrial, Inc.
• Inventors: Robinson, James V.
• Primary Examiner(s): Wityshyn, Michael G.
• Assistant Examiner(s): GITOMER, RALPH J
• Application Number:
  US08/081,644
• Time in Patent Office: 642 Days
• Field of Search: 252/380, 252/397, 252/400.52, 252/408.1, 422/17, 422/36, 424/3, 435/4, 436/63, 436/174, 436/176
• US Class Current: 435/4
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: Y10T 436/2525 Stabilizing or preserving

Abstract

A fixative composition for light microscopy including an aqueous solution of glutaraldehyde, trichloroacetic acid, a metallic salt and a surfactant is disclosed.

Claims

1. A histological composition for preparing tissue samples for examination by light microscopy consisting essentially of an aqueous solution of glutaraldehyde, trichloroacetic acid, a metallic salt and an anionic surfactant.

2. The fixative composition of claim 1 wherein the amount of glutaraldehyde on an active basis ranges from about 0.75 to about 4% by volume of the overall composition.

3. The fixative composition of claim 2 wherein the amount of glutaraldehyde on an active basis is about 1% by volume of the overall composition.

4. The fixative composition of claim 1 wherein the amount of trichloroacetic acid on an active basis ranges from about 0.5 to about 5% by volume of the overall composition.

5. The fixative composition of claim 4 wherein the amount of trichloroacetic acid on an active basis is about 1% by volume of the overall composition.

6. The fixative composition of claim 1 wherein the metallic salt is selected from the group consisting of zinc sulfate, copper sulfate, barium sulfate, cadmium sulfate, barium chloride, potassium chloride, mercuric chloride and lead chloride.

7. The fixative composition of claim 6 wherein the metallic salt is zinc sulfate.

8. The fixative composition of claim 7 wherein the amount of zinc sulfate on an active basis ranges from about 0.75 to about 2.5% by volume of the overall composition.

9. The fixative composition of claim 8 wherein the amount of zinc sulfate on an active basis is about 1% by volume of the overall composition.

10. The fixative composition of claim 1 wherein the anionic surfactant is non-foaming.

11. The fixative composition of claim 10 wherein the amount of the anionic surfactant ranges from about 0.001 to about 0.01% by volume of the overall composition.

12. The fixative composition of claim 11 wherein the amount of the anionic surfactant is about 0.0025% by volume of the overall composition.

13. A histological composition for preparing tissue samples for examination by light microscopy consisting essentially of:

on an active basis, from about 0.75 to 4% by volume glutaraldehyde, from about 0.5 to 5% by volume trichloroacetic acid, from about 0.75 to 2.5% by weight zinc sulfate and from about 0.001 to 0.01% by volume of a non-foaming anionic surfactant.