Device for conducting diagnostic assays

• US 5,401,637 A
• Filed: 05/06/1993
• Issued: 03/28/1995
• Est. Priority Date: 08/29/1990
• Status: Expired due to Fees

• Current Assignee: Stratecon Diagnostics International
• Original Assignee: Stratecon Diagnostics International
• Inventors: Pocock, Douglas A.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Trembley, Theresa A.
• Application Number:
  US08/058,496
• Time in Patent Office: 691 Days
• Field of Search: 435/7.1, 435/311, 435/810, 435/975, 435/287, 436/169, 436/174, 436/177, 436/178, 422/56, 422/58, 422/61, 422/101, 210/323.1, 210/406
• US Class Current: 435/287.7
• CPC Class Codes: C12M 23/04 Flat or tray type, drawers C12M23/10, C12M23/12, C12M23/16 take precedence
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: G01N 33/50 Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 435/975 Kit
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A diagnostic device for conducting enzymatic and chromogenic assays, the device having a plurality of test conduits therein, individual assay membrane assemblies disposed in each of the conduits, barrier walls extending between adjoining conduits to proximate a bottom wall of the device, to discourage liquid passing from one of the conduits to an adjoining conduit.

Claims

1. A diagnostic device for conducting enzymatic and chromogenic assays, said device comprising only one top housing connected to only one bottom housing, said top housing having a plurality of rectangularly-shaped openings adjacent each to another in a top surface thereof, planar wall means defining said openings and depending from said top surface to define conduits extending from said top surface completely through said top housing and in part beyond a bottom plane of said top housing, said depending wall means including top housing planar side walls and a planar barrier wall disposed between each adjacent two of said conduits, assay means disposed in said top housing in at least one of said conduits, said assay means being at least in part of a material permitting flow therethrough of a liquid substance undergoing test, an assay means horizontal support member extending side-to-side and end-to-end in said top housing, said support member being parallel to said bottom plane of said top housing and nearer to said bottom plane than to said top housing top surface, said support member having orifices therein, each of said orifices being disposed in one of said conduits, said barrier wall extending beyond said bottom plane of said top housing, said bottom housing comprising a bottom wall and upstanding side walls, edges of said depending side walls of said top housing being joined to an inclined interior surface portion of said bottom housing upstanding side walls, a distal edge of said barrier wall being proximate said bottom wall, and means for reducing pressure in said bottom housing to draw said liquid through said conduits and said assay means and into said bottom housing, said barrier wall discouraging movement of said liquid from each of said conduits.

2. The diagnostic device in accordance with claim 1, further comprising an absorbent material disposed on said bottom wall of said bottom housing, said distal edge of said barrier wall being adjacent said absorbent material, said absorbent material further discouraging movement of said liquid from said conduits.

3. The diagnostic device in accordance with claim 1 in which at least one of the top housing barrier walls and a portion of said bottom housing upstanding side walls form a reservoir chamber at a lower end of each of said conduits, each adjacent two of the reservoirs chambers being separated by one of said barrier walls.

4. The diagnostic device in accordance with claim 1, in which said assay means comprise a membrane assembly disposed in each of said conduits, said membrane assembly being fixed in said conduit in position abutting said assay means horizontal support member, said membrane assembly comprising a first plate member having a first orifice therethrough, a second plate member having a recess therein and a second orifice in said recess and extending through said second plate member, said first and second orifices being substantially in alignment with each other and with one of said support member orifices, and membrane means disposed in said second plate member recess, said first and second plate members being locked together with said membrane means disposed between said first and second orifices.

5. The diagnostic device in accordance with claim 4, further comprising retaining ridge means upstanding from said second plate to define said recess, a free edge of said retaining ridge means engaging said first plate member peripherally of said membrane means.

6. The diagnostic device in accordance with claim 5, further comprising retaining wall means depending from said first plate member peripherally of said first orifice, a free edge of said retaining wall means engaging said membrane means.

7. A method for assembling an assay device, said method comprising providing only one top housing having a row of adjacent rectangularly-shaped openings in a top surface thereof, providing planar walls depending from said top surface to define said openings and to define conduits extending completely through said top housing and, in part, beyond a bottom plane of said top housing, said walls including a planar barrier wall between each adjacent two of said conduits and including top housing planar side walls, said barrier walls extending beyond said bottom plane of said top housing, providing said bottom plane of said top housing, providing only one bottom housing including a reservoir portion comprising a bottom wall and inclined side walls upstanding therefrom, joining free edges of said top housing side walls to said bottom housing inclined side wall, said barrier walls extending into said reservoir portion with distal endel of said barrier walls being proximate said bottom wall to provide a reservoir chamber for each of said conduits, providing a membrane assembly holder comprising a horizontal support member extending side-to-side and end-to-end in said top housing, said support member being parallel to said bottom plane of said top housing and nearer to said bottom plane tan to said top housing top surface, said support member having orifices therein, each of said orifices being disposed in one of said conduits, and placing a membrane assembly in at least one of said conduits on said membrane assembly holder.