Method of preparing Limulus amoebocyte lysate
First Claim
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1. A method of preparing limulus amoebocyte lysate substantially free from factor G which comprises bringing limulus amoebocyte lysate into contact with an insoluble carrier on which a (1→
- 3)-β
-D-glucoside structural portion represented by the following formula (I) produced by depolymerizing and/or fractionating a carbohydrate chain is immobilized by a method which comprises epoxy-activating an insoluble carrier with epichlorohydrin or bisoxiranes, reacting the resulting epoxy-activated insoluble carrier with hydrazine hydrate or a dihydrazide compound to obtain a hydrazine derivative or a dihydrazide derivative and bringing the (1→
3)-β
-D-glucoside into contact with the hydrazine or dihydrazide derivative;
##STR10## wherein n represents an integer of 2 to 370.
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Abstract
Disclosed is a method of preparing limulus amoebocyte lysate substantially free from factor G which comprises bringing limulus amoebocyte lysate into contact with an insoluble carrier on which a (1→3)-β-D-glucoside structural portion represented by the following formula [I] produced by depolymerizing and/or fractionating a carbohydrate chain is immobilized: ##STR1## wherein n represents an integer of 2 to 370.
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Citations
5 Claims
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1. A method of preparing limulus amoebocyte lysate substantially free from factor G which comprises bringing limulus amoebocyte lysate into contact with an insoluble carrier on which a (1→
- 3)-β
-D-glucoside structural portion represented by the following formula (I) produced by depolymerizing and/or fractionating a carbohydrate chain is immobilized by a method which comprises epoxy-activating an insoluble carrier with epichlorohydrin or bisoxiranes, reacting the resulting epoxy-activated insoluble carrier with hydrazine hydrate or a dihydrazide compound to obtain a hydrazine derivative or a dihydrazide derivative and bringing the (1→
3)-β
-D-glucoside into contact with the hydrazine or dihydrazide derivative;
##STR10## wherein n represents an integer of 2 to 370. - View Dependent Claims (2, 3, 4, 5)
- 3)-β
Specification