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Method of preparing Limulus amoebocyte lysate

  • US 5,401,647 A
  • Filed: 05/27/1992
  • Issued: 03/28/1995
  • Est. Priority Date: 09/27/1990
  • Status: Expired due to Term
First Claim
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1. A method of preparing limulus amoebocyte lysate substantially free from factor G which comprises bringing limulus amoebocyte lysate into contact with an insoluble carrier on which a (1→

  • 3)-β

    -D-glucoside structural portion represented by the following formula (I) produced by depolymerizing and/or fractionating a carbohydrate chain is immobilized by a method which comprises epoxy-activating an insoluble carrier with epichlorohydrin or bisoxiranes, reacting the resulting epoxy-activated insoluble carrier with hydrazine hydrate or a dihydrazide compound to obtain a hydrazine derivative or a dihydrazide derivative and bringing the (1→

    3)-β

    -D-glucoside into contact with the hydrazine or dihydrazide derivative;

    ##STR10## wherein n represents an integer of 2 to 370.

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