DNA complexes with dyes designed for energy transfer as fluorescent markers

• US 5,401,847 A
• Filed: 01/27/1993
• Issued: 03/28/1995
• Est. Priority Date: 03/14/1990
• Status: Expired due to Term

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Benson, Scott C., Glazer, Alexander N.
• Primary Examiner(s): Parr, Margaret
• Assistant Examiner(s): CAMPBELL, EGGERTON A
• Application Number:
  US08/009,704
• Time in Patent Office: 790 Days
• Field of Search: 536/25.32, 536/26.6, 549/223, 546/107, 546/108, 546/176, 546/109, 204/182.8
• US Class Current: 546/107
• CPC Class Codes: C07D 221/12 Phenanthridines
• CPC Class Codes: C07D 405/12 linked by a chain containing hetero atoms as chain links
• CPC Class Codes: C07D 417/06 linked by a carbon chain containing only aliphatic carbon atoms
• CPC Class Codes: C07D 417/14 containing three or more hetero rings
• CPC Class Codes: C09B 23/04 one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 2565/102 Multiple non-interacting labels
• CPC Class Codes: C12Q 2565/1025 labels being on the same oligonucleotide
• CPC Class Codes: G01N 27/44726 using specific dyes, markers or binding molecules
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture

Abstract

Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.

Claims

1. A fluorescent heteromultimer which is thiazole orange-thiazole blue dimer of formula 7 of FIG. 2.

2. A fluorescent heteromultimer which is thiazole orange-ethidium dimer of formula 11 of FIG. 3.