Biocompatible, low protein adsorption affinity matrix

• US 5,403,750 A
• Filed: 04/08/1991
• Issued: 04/04/1995
• Est. Priority Date: 03/06/1991
• Status: Expired due to Fees

• Current Assignee: Millipore Investment Holdings, Ltd. (Merck & Co., Inc.)
• Original Assignee: W R Grace & Company - CONN (Standard Industries Holdings, Inc.)
• Inventors: Heifetz, Aaron H., Braatz, James A.
• Primary Examiner(s): Acquah, Samuel A.
• Application Number:
  US07/682,502
• Time in Patent Office: 1,457 Days
• Field of Search: 528/48, 528/52, 528/53, 528/59, 528/904, 427/2, 427/207.1, 427/221, 427/435, 210/500.24, 428/423.1, 428/423.9, 428/424.2, 428/424.6, 428/425.1, 428/425.5, 428/425.6, 604/8, 604/19, 604/403, 435/174, 435/176, 435/181, 435/182, 525/403, 525/418, 525/420, 525/424, 525/454, 436/531, 436/120, 436/128, 436/131
• US Class Current: 436/531
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 33/068 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds A61L33/062 takes precedence
• CPC Class Codes: B01D 15/3809 of the antigen-antibody type, e.g. protein A, G, L chromatography
• CPC Class Codes: B01J 20/103 comprising silica
• CPC Class Codes: B01J 20/20 comprising free carbon; comprising carbon obtained by carbonising processes
• CPC Class Codes: B01J 20/262 obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds per se C08G
• CPC Class Codes: B01J 20/28004 Sorbent size or size distribution, e.g. particle size
• CPC Class Codes: B01J 20/28016 Particle form
• CPC Class Codes: B01J 20/28078 Pore diameter
• CPC Class Codes: B01J 20/285 based on polymers
• CPC Class Codes: B01J 20/286 Phases chemically bonded to a substrate, e.g. to silica or to polymers
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3227 by end-capping, i.e. with or after the introduction of functional or ligand groups
• CPC Class Codes: B01J 20/3272 Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: B01J 2220/56 Use in the form of a bed
• CPC Class Codes: B01J 2220/58 Use in a single column
• CPC Class Codes: C08G 18/10 Prepolymer processes involving reaction of isocyanates or isothiocyanates with compounds having active hydrogen in a first reaction step
• CPC Class Codes: C08G 18/283 Compounds containing ether groups, e.g. oxyalkylated monohydroxy compounds
• CPC Class Codes: C08G 18/302 Water
• CPC Class Codes: C08G 18/4825 : Polyethers containing two hydroxy groups C08G18/4833 - C08G18/5096 take precedence
• CPC Class Codes: C08G 18/4829 : Polyethers containing at least three hydroxy groups C08G18/4833 - C08G18/5096 take precedence
• CPC Class Codes: C08G 18/4833 : Polyethers containing oxyethylene units
• CPC Class Codes: C08G 18/4837 : and other oxyalkylene units
• CPC Class Codes: C08G 18/485 : containing mixed oxyethylene-oxypropylene or oxyethylene-higher oxyalkylene end groups
• CPC Class Codes: C08G 18/755 : and at least one isocyanate or isothiocyanate group linked to a secondary carbon atom of the cycloaliphatic ring, e.g. isophorone diisocyanate
• CPC Class Codes: C08G 2210/00 : Compositions for preparing hydrogels
• CPC Class Codes: C08L 75/04 : Polyurethanes
• CPC Class Codes: C12N 2533/30 : Synthetic polymers
• CPC Class Codes: C12N 5/0068 : General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: G01N 33/54313 : the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/54393 : Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: G02B 1/043 : Contact lenses
• CPC Class Codes: Y10S 528/904 : Isocyanate polymer having stated hydrophilic or hydrophobic property
• CPC Class Codes: Y10T 428/31551 : Of polyamidoester [polyurethane, polyisocyanate, polycarbamate, etc.]
• CPC Class Codes: Y10T 428/31569 : Next to natural rubber
• CPC Class Codes: Y10T 428/31573 : Next to addition polymer of ethylenically unsaturated monomer
• CPC Class Codes: Y10T 428/3158 : Halide monomer type [polyvinyl chloride, etc.]
• CPC Class Codes: Y10T 428/31591 : Next to cellulosic
• CPC Class Codes: Y10T 428/31598 : Next to silicon-containing [silicone, cement, etc.] layer
• CPC Class Codes: Y10T 428/31601 : Quartz or glass
• CPC Class Codes: Y10T 436/182 : Organic or sulfhydryl containing [e.g., mercaptan, hydrogen, sulfide, etc.]
• CPC Class Codes: Y10T 436/201666 : Carboxylic acid
• CPC Class Codes: Y10T 436/203332 : Hydroxyl containing

Abstract

Affinity matrices useful for the chromatography and immobilization of biological materials and the method of preparing and using the same are disclosed. The affinity supports are based on hydrated polyurethane polymers which have been activated to provide a means for covalently attaching a variety of bioaffinity agents. The hydrated polymer matrices are characterized by their biocompatibility and resistance to nonspecific protein adsorption. Preferably, the prepolymers used to prepare the hydrated polymers are isocyanate-capped oxyethylene-based diols or polyols, at least 75% of said diols and polyols having a molecular weight of 7000 to about 30,000.

Claims

1. An affinity matrix for chromatography and immobilization of biologically active materials comprising a support having a polyurethane coating thereon of said polymer thereon, wherein said polyurethane polymer is derived from prepolymer units of oxyethylene based alcohols having essentially all of the hydroxyl groups capped with polyisocyanate groups and having at least 15% of said polyisocyanate groups reacted with a modifying compound having a first NCO reactive group and a second functional group having a substantially less NCO reactive group, and wherein said polyurethane polymer has been activated by converting said second functional groups to an active functional group capable of covalently attaching a bioaffinity agent, said matrix being characterized by a biocompatible surface which resists nonspecific protein adsorption, wherein the modifying compound is selected from the group consisting of ethanolamine;

aminoethyl hydrogensulfate;

taurine;

4-aminosulfonyl-1-hydroxy-2-napththoic acid;

glucosamine;

5-(aminosulfonyl) N-((1-ethyl-2-pyrrolidinyl)methyl)-2-methoxybenzamide;

sulfamylphenyl-D-glucosylamine;

4-carboxybenzene-sulfonamide;

sulfanilamide;

cyclic-adenosine monophosphate;

2-aminoethyl phosphonic acid;

tyrosine;

tyramine;

dibutylamine;

L- or DL-cysteine;

L- or DL-cysteine ethyl ester;

L- or DL-cystine dimethyl ester;

L- or DL-Cystine;

L- or DL-cysteinesulfonic acid;

L- or DL-cysteic acid;

cystamine;

2-mercaptoethanol;

ethanethiol;

glutathione;

3-amino-1,2-propanediol;

3-amino-1-propane sulfonic acid;

3-aminophenyl boronic acid;

2-amino-2-deoxy-D-galactose;

1-amino-1-deoxy-D-galactose;

p-aminophenyl-alpha-D-glucose;

p-aminophenyl-1-thio-beta-D-galactose;

penicillamine;

peptides with sulfhydryl groups;

peptides with free amino groups;

animal hormones;

polysaccharides;

lipids;

nucleic acids;

amino sugars;

amino acids;

amine surfactants;

diamines and polyamines.

2. The matrix of claim 1 in which substantially all of said prepolymer units are oxyethylene-based diols or polyols having an average number molecular weight of about 7000 to about 30,000.

3. The matrix of claim 1 in which at least 75% of said prepolymer units are oxyethylene-based diols and polyols having an average number molecular weight of about 7000 to about 30,000.

4. The matrix of claim 2 in which said diols or polyols are capped with an aliphatic or cycloaliphatic polyisocyanate.

5. The matrix of claim 1 in which said polyisocyanate is isophorone diisocyanate or methylene bis(cyclohexyl diisocyanate).

6. The matrix of claim 1 in which up to 100% of the isocyanate groups of the prepolymer are reacted with the modifying compound.

7. The matrix of claim 1 in which the polyurethane polymer has been activated to convert essentially all of the second functional groups to an active functional group capable of covalently attaching a bioaffinity agent.

8. The matrix of claim 1 in which the polyurethane polymer has been activated to convert substantially all of the second functional groups to an active functional group capable of covalently attaching a bioaffinity agent.

9. The matrix of claim 1 in which the support is in the form of a microporous membrane or a particulate media.

10. The matrix of claim 9 in which the support is silica gel.

11. The matrix of claim 9 in which the support is charcoal.

21. An affinity chromatographic device comprised of the matrix of claim 1.

22. An immobilized bioaffinity agent comprising a bioaffinity agent covalently attached to the matrix of claim 1.

23. An extracorporeal therapeutic device comprised of the immobilized bioaffinity agent of claim 22.

24. A purification or isolation system for a biologically-active ligand or binder molecule, said system comprising the immobilized bioaffinity agent of claim 22, wherein the bioaffinity agent is capable of selectively binding said ligand or binder molecule.

25. A diagnostic assay system comprising the affinity matrix of claim 1 having a bioaffinity agent covalently attached thereto, and further comprising a holder for said matrix.

12. The process of preparing an affinity matrix for chromatography or immobilization of biological materials, which matrix is characterized by a biocompatible surface which resists nonspecific protein adsorption, said process comprising the steps of:

a) preparing a prepolymer, the units of which are oxyethylene-based alcohols having essentially all of the hydroxyl groups capped with polyisocyanate;

b) reacting said polyisocyanate capped prepolymer with a modifying compound having a first functional group which is NCO reactive and a second functional group which is substantially less NCO reactive to form a modified prepolymer;

c) coating the modified prepolymer onto a support;

d) curing the modified prepolymer with water to form a modified polyurethane polymer characterized by the second functional group inserted in the prepolymer; and

e) reacting the modified polyurethane polymer with an activating compound to convert the second functional group of said polymer to an active functional group capable of covalently attaching a bioaffinity agent.

13. The process of claim 12 in which a large molar excess of said activating compound is used.

14. The process of claim 13 in which essentially all of the second functional groups of the modified polyurethane polymer is converted to an active functional group capable of covalently attaching a ligand or binder molecule.

15. The process of claim 13 in which substantially all of the second functional groups of the modified polyurethane polymer is converted to an active functional group capable of covalently attaching a ligand or binder molecule.

16. The process of claim 13 in which the modified polyurethane polymer is reacted with the activating compound in a nonaqueous environment.

17. The process of claim 13 in which the prepolymer is coated onto a support prior to curing with water.

18. The process of claim 13 in which the prepolymer units are oxyethylene based diols and polyols which are capped with an aliphatic polyisocyanate.

19. The process of claim 13 in which about 15% to about 30% of the prepolymer isocyanate groups are modified.

20. The process of claim 13 in which the prepolymer is coated onto a silica or charcoal prior to curing with water.