Method of sequencing DNA
First Claim
Patent Images
1. A method for solid-phase sequencing of one strand of double-stranded DNA comprising the steps of:
- (A) providing said double-stranded DNA;
(B) immobilizing on a solid support one terminus of one of the two strands of said double-stranded DNA;
(C) subjecting said immobilized double-stranded DNA to strand separation, thereby providing an immobilized strand and an unattached strand;
(D) removing said unattached strand, thereby providing an immobilized single-stranded DNA;
(E) sequencing said immobilized single-stranded DNA.
1 Assignment
0 Petitions
Accused Products
Abstract
The invention relates to a method of sequencing target DNA in which said DNA is provided in double stranded form immobilized on a solid support via one terminus of one of the two strands thereof and is then subjected to strand separation whereby the unattached strand is removed prior to sequencing the immobilized strand. The target DNA, e.g genomic DNA, may initially be amplified by the PCR method using at least one oligonucleotide primer which is either attached to a solid support or carries means for attachment to a solid support.
165 Citations
13 Claims
-
1. A method for solid-phase sequencing of one strand of double-stranded DNA comprising the steps of:
-
(A) providing said double-stranded DNA; (B) immobilizing on a solid support one terminus of one of the two strands of said double-stranded DNA; (C) subjecting said immobilized double-stranded DNA to strand separation, thereby providing an immobilized strand and an unattached strand; (D) removing said unattached strand, thereby providing an immobilized single-stranded DNA; (E) sequencing said immobilized single-stranded DNA. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
-
Specification