Electrochemical sensor with multiple zones on a disc and its application to the quantitative analysis of glucose

• US 5,407,554 A
• Filed: 04/08/1994
• Issued: 04/18/1995
• Est. Priority Date: 05/10/1993
• Status: Expired due to Fees

• Current Assignee: Asulab SA (The Swatch Group AG)
• Original Assignee: Asulab SA (The Swatch Group AG)
• Inventors: Saurer, Eric
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): Bell, Bruce F.
• Application Number:
  US08/224,778
• Time in Patent Office: 375 Days
• Field of Search: 204/403, 204/412, 204/416, 204/418, 204/419, 435/291, 435/817, 422/68.1, 422/82.03
• US Class Current: 204/403.03
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An electrochemical sensor having multiple measuring zones (34) that are sequentially insulatable, disposed on the circumference of an insulating disc (1) and associated with each other by peripheral current collectors (16, 17) which extend radially inward up to central zones where they form contacts (23, 24) for a measuring apparatus. The disc-sensor also has a device (6) for rotating the disc, and guide marks for positioning (11) and for isolating (7) the measuring zones (34), and may be contained in a housing (40). The invention is useful in the quantitative analysis of glucose.

Claims

1. A disposable electrochemical sensor having multiple measuring zones that are sequentially separable, and using a single sensor which is introduced into a measuring apparatus for carrying out several successive measurements of the same physico-chemical characteristic or of a concentration of the same component present in a solution, an effluent or a fluid, said sensor comprising:

a thin, insulating rigid support in the shape of a disc;

at least two current collectors of identical or different conducting materials arranged on the disc to be insulated from each other and each having portions or extensions situated in a peripheral border adjacent to the periphery of the disc, said portions or extensions forming electrodes in the measuring zones connected to each other by a corresponding one of said current collectors, and each of said current collectors extending up to a zone close to the center of the disc where said current collector has a terminal part shaped to provide a contact zone;

drive means for rotating the disc; and

guide means for positioning the measuring zones in the measuring apparatus and for isolating a used measuring zone from the other, still unused, measuring zones.

2. A sensor according to claim 1, wherein the two current collectors are supported by the same face of the disc and are shaped as two concentric rings disposed inside the peripheral border, the ring located closest to the center of the disc is open, and each ring has a radial extension directed towards the center of the disc to provide a contact zone.

3. A sensor according to claim 2, which has a third current collector having a concentric portion close to the two first collectors and a radial extension directed towards the center of the disc to provide a third contact zone.

4. A sensor according to claim 1, wherein the two current collectors are supported by the same face of the disc and are shaped as two closed concentric rings arranged inside the peripheral border, the ring located closest to the center of the disc having a radial extension supported by the same face of the disc and directed towards the center thereof and the ring closest to the edge of the disc having a radial extension supported by the other face of the disc and joined to said edge ring by a current collector portion traversing the disc.

5. A sensor according to claim 4, which has a third current collector having a concentric portion close to the two first collectors and a radial extension directed towards the center of the disc to provide a third contact zone.

6. A sensor according to claim 1, wherein the two current collectors are supported respectively by each face of the disc and are each shaped like a star, each branch of said star emanating from a disc or from a central crown forming a contact zone and extending up to the edge of the disc.

7. A sensor according to claim 6, wherein the measuring zones have traversing orifices assisting by capillarity the ionic junction of the electrodes carried by each face of the disc.

8. A sensor according to claim 1, wherein the contact zones are in the shape of a pastille, a disc or an open or a closed ring situated on the same face of the disc or respectively on each face of it.

9. A sensor according to claim 1, wherein the measuring zones are regularly distributed on the disc in all or part of substantially equal circular sectors, and wherein the perimeter of said measuring zones is delimited, either by a deformation of the insulating support disc, or by windows in an insulating film covering the disc.

10. A sensor according to claim 1, wherein the current collectors are composed of bands obtained by thin or thick layer deposition of a conducting material or by lamination of a covered insulating film of a conducting material, and said conducting material is gold, silver, platinum, nickel, palladium, titanium or carbon.

11. A sensor according to claim 10, wherein one current collector is of platinum and constitutes a working electrode, and wherein the other current collector is of silver/silver chloride and constitutes a reference electrode.

12. A sensor according to claim 11, wherein the working electrode is coated with a reagent having at least one enzyme specific to the component present in the solution.

13. A sensor according to claim 12, wherein the reagent has a mediator.

14. A sensor according to claim 1, wherein the rigid insulating material constituting the disc is a vinyl resin, a ceramic, mica, a polyethylene terephthalate or a glass.

15. A sensor according to claim 1, wherein the drive means comprises a central polygonal traversing hole, a toothed crown for driving the disc by means of gears or friction and located on one of its faces, or indentations at the edge of the disc.

16. A sensor according to claim 1, wherein the guide means for the measuring zones comprises recesses regularly spaced in the thickness of the circumference of the disc.

17. A sensor according to claim 1, wherein the disc has on one of its faces a portion of a radial rib arranged to cooperate with an abutment located in the measuring apparatus to prevent the disc from rotating more than 360°

.

18. A sensor according to claim 1, wherein the disc has an anti-return catch.

19. A sensor according to claim 1, wherein the disc has a thickness between 0.5 and 6 mm and a diameter between 5 and 15 cm, and wherein the sensor comprises between 6 and 24 of said measuring zones.

20. A sensor according to claim 1, which has a housing provided with openings giving access to at least one of said measuring zones and to an associated isolating zone, to the contact zones and to the drive means, and said housing is a protection means and a positioning means of the sensor in the measuring apparatus.