Biosensor containing a biochemical substance immobilized on a layer of olefinic-unsaturated, epoxy functional cross-linked polysiloxane

• US 5,407,818 A
• Filed: 03/22/1993
• Issued: 04/18/1995
• Est. Priority Date: 03/23/1992
• Status: Expired due to Fees

• Current Assignee: Siemens AG
• Original Assignee: Siemens AG
• Inventors: Formanek, Helmut, Feucht, Hans-Dieter, Wanner, Gerhard, von Gentzkow, Wolfgang
• Primary Examiner(s): Naff, David M.
• Application Number:
  US08/035,030
• Time in Patent Office: 757 Days
• Field of Search: 435/174, 435/176, 435/180, 435/182, 435/288, 435/12, 435/14, 435/25, 435/26, 435/27, 204/403, 528/25
• US Class Current: 435/180
• CPC Class Codes: C12Q 1/002 Electrode membranes

Abstract

A biosensor is prepared having a selective detection system containing a biochemical substance such as an enzyme immobilized on an olefinic-unsaturated, epoxyfunctional polysiloxane. Prior to immobilization of the biochemical substance, the polysiloxane is applied as a layer to a carrier and cross-linked by treatment with high-energy radiation. A biochemical substance is reacted with epoxy groups of the cross-linked polysiloxane. Any non-reacted epoxy groups are reacted with a compound containing an amino group, a carboxyl group or an amino group and a carboxyl group to stabilize. After cross-linking and before reacting of the biochemical substance, the cross-linked polysiloxane can be hydrophilized by reacting some of the epoxy groups with a hydrophilic compound containing a reactive group.

Claims

1. A biosensor prepared by a method comprising the steps of:

applying an olefinic-unsaturated, epoxyfunctional polysiloxane to a carrier material in the form of a layer, the polysiloxane having the following structure;

##STR7## where the following applies;

E= ##STR8## Z=a vinyl group or a photopolymerizable group with 8 to 40 C. atoms, which is obtained by addition of a photopolymerizable compound to E, and subsequent addition of an aliphatic, cycloaliphatic or aromatic monoisocyanate or monoisothiocyanate with 2 to 10 C. atoms to the secondary OH group formed upon opening of the epoxide ring,

R¹ =alkyl with 1 to 4 C. atoms or phenyl,

R² =R¹, E or Z,

where R¹ and R² can be the same or different in each instance,

x=50 to 1000, y=10 to 300, z=3 to 8,

cross-linking the polysiloxane by means of high-energy radiation to form an epoxyfunctional cross-linked polysiloxane polymer matrix,

treating the layer with an aqueous solution of a biochemical substance having groups that covalently react with the epoxy groups of the polysiloxane, whereby the biochemical substance is immobilized in the polymer matrix by reaction with epoxy groups, and

stabilizing the layer by reaction of any non-reacted epoxy groups remaining after the step of treating with a compound containing an amino group, a carboxyl group or an amino group and a carboxyl group.

2. The biosensor according to claim 1 wherein the cross-linked polysiloxane is hydrophilized before immobilization of the biochemical substance by reacting some of the epoxy groups of the polysiloxane with a hydrophilic compound containing a reactive group.

3. The biosensor according to claim 1 wherein the biochemical substance is an enzyme.