Liquid specimen transfer apparatus and method

• US 5,411,065 A
• Filed: 01/10/1994
• Issued: 05/02/1995
• Est. Priority Date: 01/10/1994
• Status: Expired due to Fees

• Current Assignee: Pyxis Corp. (Cardinal Health, Inc.)
• Original Assignee: KVM Technologies, Inc. (Cardinal Health, Inc.)
• Inventors: Miller, Thomas G., Donnelly, Sean M., Meador, James W., Waters, Louis A. Jr., Nikirk, Christopher T.
• Primary Examiner(s): Recla, Henry J.
• Assistant Examiner(s): Douglas, Steven O.
• Application Number:
  US08/179,436
• Time in Patent Office: 477 Days
• Field of Search: 141/1, 141/25, 141/26, 141/27, 141/81, 141/98, 141/114, 141/117, 141/130, 422/63, 436/180, 222/214, 222/81, 222/82, 222/152, 73/863.81, 73/863.83, 73/863.84
• US Class Current: 141/1
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 33/487 of liquid biological material
• CPC Class Codes: G01N 35/1009 Characterised by arrangements for controlling the aspiration or dispense of liquids
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A method of obtaining aliquots or samples from a liquid specimen collection container and an apparatus which automateally perforths those methods involves transferring a sample of liquid from a sealed non-deformingly expandable container containing gas and the liquid without contaminating the liquid remaining inside the container, and comprises (a) non-deformingly volumetrically expanding the sealed container sufficiently to reduce pressure therein below ambient pressure outside the container, (b) applying heat selectively to a locus on said enlarged container to non-invasively create a hole having a diameter effective to equilibrate the pressure inside the container with said ambient pressure without loss of liquid from the container, and (c) non-deformingly volumetrically reducing the container to expel liquid from the container through the hole.

Claims

1. A method of transferring a sample of liquid from a sealed non-deformingly expandable container containing gas and the liquid without invasively contacting the liquid remaining inside the container, said method comprising:

(a) non-deformingly volumetrically expanding said sealed container sufficiently to reduce pressure therein below ambient pressure outside the container,

(b) applying heat selectively to a locus on said container to non-invasively create a hole having a diameter effective to equilibrate the pressure inside the container with said ambient pressure without loss of liquid from the container, and

(c) non-deformingly volumetrically reducing said container to expel liquid from the container through said hole.

2. The method of claim 1 in which step (b) includes creating said hole with a laser beam.

3. The method of claim 1 in which said step (b) includes creating said hole with a heated gas stream column.

4. The method of claim 1 in which said hole has a diameter effective in said container to provide surface tension at the hole greater than the hydrostatic pressure within the enlarged container.

5. The method of claim 1 in which said locus is at a bottom portion of the container when the hole is being created.

6. The method of claim 1 in which the container includes a projection from a bottom portion thereof, said projection having a duct opening into the container and having a closure closing said duct remotely from said duct opening, and wherein said locus is at said closure.

7. A method of transferring an aqueous liquid specimen from a sealed non-deformingly expandable container containing gas and a liquid without invasively contacting liquid inside the container, such container having a bottom portion including at least one nozzle portion dependent therefrom, said nozzle having a duct opening into the container and a closure closing said duct remotely from said duct opening, said method comprising:

(a) placing said liquid specimen container and a receptacle for the liquid specimen so that the container is in a dispense station above said receptacle,

(b) non-deformingly volumetrically expanding said container sufficiently to reduce pressure therein below ambient pressure outside the container,

(c) applying heat selectively to a locus on said duct closure to non-invasively create a hole in the closure having a diameter effective to equilibrate the pressure inside the container with said ambient pressure without loss of liquid from the container, and

(d) non-deformingly reducing the volume of said container to expel liquid from the container through said hole into said receptacle.

8. The method of claim 7 wherein said said nozzle is not over said receptacle when step (c) is conducted, and further comprising locating said nozzle over said receptacle before said step (d) is conducted.

9. The method of claim 8 further comprising locating said receptacle so that the bottom of said nozzle is below the top of said receptacle.

10. The method of claim 7 further comprising, after step (d), applying heat selectively to the periphery of said locus to melt close said hole.

11. The method of claim 7 in which said container has a readable identification code and said receptacle contains a readable identification code, and wherein said method further comprises:

reading said code of a container and said code of a receptacle, for correlating the code identification of a container from which liquid is expelled to the code identification of the receptacle into which the liquid is expelled.

12. The method of claim 11 in which said step of reading includes rotating the container past a code reader, and wherein said method further comprises:

determining the position of said nozzle, and moving said nozzle to a position for conduct of said step of opening.

13. The method of claim 11 in which said step (a) is further characterized in that said receptacle is among a plurality of receptacles in a holder and said container is among a plurality of containers in a rack, and wherein said rack has a readable identification code, said method further comprising correlating the code identification of the rack to said code identification of each said receptacle receiving liquid from a container in said rack.

14. The method of claim 7 in which said step (a) is further characterized in that said receptacle is among a plurality of receptacles in a holder and said container is among a plurality of containers in a rack, and wherein said placing includes advancing said holder and said rack in intersecting directions to place said container in said dispense station above said receptacle, and further comprising:

after said step (d), advancing said holder and said rack to place another container in said dispense station above another said receptacle, and

conducting said steps (b), (c) and (d) on said other container, said receptacle in step (d) being said other receptacle.

15. The method of claim 7 further comprising:

forming an input queue of a plurality of said holders, and

feeding said holders from said input queue for advancement in said first direction.

16. The method of claim 15 in which said input queue is transverse to said first direction.

17. The method of claim 7 in which after a predetermined one of said receptacles in said holder receives liquid, said holder is advanced in said first direction from below said dispense station to an output position.

18. The method of claim 17 further comprising:

receiving a holder at said output position, and

forming an output queue of a plurality of said received holders.

19. The method of claim 18 in which said output queue is transverse to said first direction.

20. A method of transferring to a receptacle an aliquot of an aqueous liquid specimen from a sealed non-deformingly expandable container containing gas and a liquid,

said container having a bottom portion including at least one nozzle portion dependent therefrom, said nozzle having a duct opening into the container and a closure closing said duct remotely from said duct opening,

said container having a readable identification code on the exterior thereof and being one of a plurality of containers in a rack, said rack having a readable identification code and including a code structure adjacent each container for identification of the location of the container in the rack,

said receptacle having a readable identification code on the exterior thereof and being one of a plurality of receptacles in a holder,

said method comprising:

(a) feeding a first said holder for advancement in a first direction to place a first said receptacle below a dispense station,

(b) reading said code of said first receptacle,

(c) reading the rack code identification, for correlating the code identification of the rack to said code identification of the receptacle in said holder

(d) feeding a first said rack in a second direction transverse to said first direction to place said first container at said dispense station above said first receptacle,

(e) rotating said first container past (i) a code reader and reading said code of said container, for correlating the code identification of the container at the dispense station to the code identification of said first receptacle below the dispense station, and past (ii) a nozzle detecting sensor for determining the position of a said nozzle,

(f) determining the position of a said nozzle on said first container and rotating said container at said dispensing station to position said nozzle at a nozzle opening position,

(g) non-deformingly volumetrically expanding said first container sufficiently to reduce pressure therein below ambient pressure outside the container,

(h) applying heat selectively to a locus on said closure to non-invasively create a hole having a diameter effective to equilibrate the pressure inside the container with said ambient pressure without loss of liquid from the container,

(i) rotating said first container at said dispensing station to position said nozzle above said first receptacle,

(j) elevating said first receptacle from said holder so that the bottom of said nozzle is below the top of said receptacle,

(k) non-deformingly reducing the volume of said first container to expel liquid from the container through said hole into said first receptacle,

(l) lowering said first receptacle to said holder so that the bottom of said nozzle is above the top of said receptacle,

(m) rotating said first container at said dispensing station to position said nozzle at a nozzle hole closing position,

(n) applying heat selectively to the periphery of said locus to melt close said hole,

(o) advancing said holder in said first direction and said rack in said second direction to place another container in said rack in said dispense station and another receptacle below said dispense station,

(p) conducting said steps (b), (c) and (e)-(n) on said other container and other receptacle,

(q) after predetermined receptacles in said holder receive liquid, advancing said holder in said first direction from said dispense station to an output position.

21. An apparatus for transferring a sample of liquid from a sealed non-deformingly expandable container containing gas and the liquid without invasively contacting the liquid remaining inside the container, said apparatus comprising in combination:

(a) means for non-deformingly volumetrically expanding and constricting said sealed container to vary volume therein, upon expansion to reduce pressure therein below ambient pressure outside the container, and upon constriction to expel liquid from the container through a hole formed in said container, and

(b) means for applying heat selectively to a locus on said container in an expanded condition to non-invasively create a hole having a diameter effective to equilibrate the pressure inside the sealed container with said ambient pressure without loss of liquid from the container and for applying heat selectively to the periphery of said locus of said container after constriction thereof to melt close said hole.

22. The apparatus of claim 21 in which said means (b) includes means for creating said hole with a laser beam.

23. The apparatus of claim 21 in which said means (b) includes means for creating said hole with a heated gas stream column.

24. The apparatus of claim 21 in which said hole has a diameter effective in said container to provide surface tension at the hole greater than the hydrostatic pressure within the container.

25. The apparatus of claim 21 in which said locus is at a bottom portion of the container when the hole is being created.

26. The apparatus of claim 21 in which the container includes a projection from a bottom portion thereof, said projection having a duct opening into the container and having a closure closing said duct remotely from said duct opening, and wherein said locus is at said closure.

27. An apparatus for transferring an aqueous liquid specimen from a sealed nondeformingly expandable container containing gas and a liquid without invasively contacting liquid inside the container, such container having a bottom portion including at least one nozzle portion dependent therefrom, said nozzle having a duct opening into the container and a closure closing said duct remotely from said duct opening, said apparatus comprising in operative combination:

(a) means for placing said liquid specimen container and a receptacle for the liquid specimen so that the container is in a dispense position above said receptacle,

(b) means for non-deformingly volumetrically expanding and constricting said sealed container to vary volume therein, upon expansion to reduce pressure therein below ambient pressure outside the container, and upon constriction to expel liquid from the container through a hole formed in said duct closure, and

(c) means for applying heat selectively to a locus on said duct closure to non-invasively create a hole in the closure having a diameter effective to equilibrate the pressure inside the container with said ambient pressure without loss of liquid from the container and for applying convective heat selectively to the periphery of said locus to melt close said hole.

28. The apparatus of claim 27 further comprising means for detecting the location of said nozzle at said dispensing station for locating said nozzle over said receptacle before creating said hole therein.

29. The apparatus of claim 27 further comprising means for locating said receptacle so that the bottom of said nozzle is below the top of said receptacle.

30. The apparatus of claim 27 in which said container has a readable identification code and said receptacle contains a readable identification code, and wherein said apparatus further comprises:

means for reading said code of a container and said code of a receptacle, for correlating the code identification of a container from which liquid is expelled to the code identification of the receptacle into which the liquid is expelled.

31. The apparatus of claim 27 further characterized in that said receptacle is among a plurality of receptacles in a holder and said container is among a plurality of containers in a rack, and wherein said means for placing includes means for advancing said holder and said rack in intersecting directions to place said container in said dispensing station below said receptacle.

32. The apparatus of claim 27 further comprising:

means for forming an input queue of a plurality of said holders, and

means for feeding said holders from said input queue for advancement in said first direction to said ready position.

33. The apparatus of claim 32 in which said input queue is transverse to said first direction.

34. The apparatus of claim 27 further comprising:

means for receiving a holder at an output position at the terminus of said first direction of advance, and

means for forming an output queue of a plurality of said received holders.

35. The apparatus of claim 34 in which said output queue is transverse to said first direction.

36. An apparatus for transferring to a receptacle an aliquot of an aqueous liquid specimen from a sealed non-deformingly expandable container containing gas and a liquid,

said container having a bottom portion including at least one nozzle portion dependent therefrom, said nozzle having a duct opening into the container and a closure closing said duct remotely from said duct opening,

said container having a readable identification code on the exterior thereof and k-ring one of a plurality of containers in a rack, said rack having a readable identification code and including a code structure adjacent each container for identification of the location of the container in the rack,

said receptacle having a readable identification code on the exterior thereof and being one of a plurality of receptacles in a holder,

said apparatus comprising in operative combination:

(a) means for feeding a first said holder for advancement in a first direction to place a first said receptacle below a dispense station,

(b) means for reading said code of said first receptacle,

(c) means for reading the rack code identification to provide a signal for use for correlating the code identification of the rack to said code identification of the receptacle in said holder,

(d) means for feeding a first said rack in a second direction transverse to said first direction to place said first container at said dispense station above said first receptacle,

(e) means for reading said code of said first container for correlating the code identification of the container at the dispense station to the code identification of said first receptacle below the dispense station,

(f) means for sensing and determining the position of a said container nozzle at said dispense station,

(g) means for rotating said first container past (i) said means for reading said code of said container and past (ii) said means for sensing the position of a said nozzle, for rotating said container at said dispensing station to position said nozzle at a nozzle opening position, for rotating said first container at said dispensing station to position said nozzle above said first receptacle, and for rotating said first container at said dispensing station to position said nozzle at a nozzle hole closing position,

(h) means for non-deformingly volumetrically expanding and constricting said sealed container to vary volume therein, upon expansion to reduce pressure therein below ambient pressure outside the container, and upon constriction to expel liquid from the container through a hole formed in said closure,

(i) means for applying heat selectively to a locus on said closure to non-invasively create a hole in the closure having a diameter effective to equilibrate the pressure inside the theretofore sealed container with said ambient pressure without loss of liquid from the container and for applying heat selectively to the periphery of said locus to melt close said hole, and

(j) means for elevating said first receptacle from said holder so that the bottom of said nozzle is below the top of said receptacle and for lowering said first receptacle to said holder so that the bottom of said nozzle is above the top of said receptacle.