Apparatus for the determination of analytes in liquid samples

• US 5,411,708 A
• Filed: 04/06/1993
• Issued: 05/02/1995
• Est. Priority Date: 08/06/1991
• Status: Expired due to Term

• Current Assignee: Pompeo Moscetta
• Original Assignee: Pompeo Moscetta
• Inventors: Moscetta, Pompeo
• Primary Examiner(s): Czaja, Donald E.
• Assistant Examiner(s): Cano, Milton I.
• Application Number:
  US08/039,101
• Time in Patent Office: 756 Days
• Field of Search: 422/68.1, 422/81, 422/82, 436/180, 436/43
• US Class Current: 422/81
• CPC Class Codes: G01N 2201/122 Kinetic analysis; determining reaction rate
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: G01N 35/1097 characterised by the valves valves in general F16K
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An apparatus for the determination of analytes in liquid samples is disclosed. The apparatus comprises in fluid connection a positive-displacement pump, a chamber in which the reagents specific for the analytes to be determined can be delivered, a detector, a second chamber and a device for the introduction of sample within the circuit and for the discharge. The apparatus also provides for apparatus to control flow and to control of the operation of each component. A process for the determination of the analytes is also disclosed.

Claims

1. Apparatus for determining analytes in liquid samples which comprises

a) means for sampling;

b) bidirectional pump means in fluid connection with said means for sampling;

c) an injection chamber in fluid connection with said pump means, through a bottom input, said injection chamber comprising a first outlet tube which draws to an inside thereof;

d) means for controlling addition of at least one reagent and calibrator into said injection chamber while in fluid connection with said injection chamber;

e) means for determining concentration of said analytes, in fluid connection with said injection chamber through said first outlet tube;

f) means for interrupting the fluid connection between said injection chamber and said means for determining the concentration of analytes;

g) an expansion chamber in fluid connection with said means for determining concentration, through an inlet tube which draws to an inside thereof, said expansion chamber comprising a second outlet tube;

h) means for discharging and recirculating the liquid samples in fluid connection with said expansion chamber through said second outlet tube and with said means for sampling and

i) means for operating and controlling the apparatus, wherein said means for controlling addition is responsive to the fluid flow from said injection chamber to said expansion chamber, whereby said apparatus operates as a single hydraulic circuit closed to the external air.

2. Apparatus according to claim 1, wherein said means for sampling is formed by a three-way valve.

3. Apparatus according to claim 2, wherein said means for sampling is connected to a switch valve outside said apparatus.

4. Apparatus according to claim 1, wherein said bidirectional pump means are constituted by a positive displacement pump.

5. Apparatus according to claim 1, wherein said injection chamber is a fixed volume chamber capable of maintaining a depressure in an inside thereof, caused by said bidirectional pump means, thereby initiating delivery of reagent or calibrator.

6. Apparatus according to claim 1, wherein in said injection chamber said bottom input and said first outlet tube are offset so that when said bidirectional pump means deliver the analytes containing liquid sample to said injection chamber, a turbulent mixing with said at least one reagent and calibrator is provoked.

7. Apparatus according to claim 6, wherein said injection chamber is provided with overflow means.

8. Apparatus according to claim 1, wherein said means for the controlling addition of at least one reagent and calibrator are constituted by a number of separate compartments, each one containing a reagent or a calibrator, connected to said injection chamber.

9. Apparatus according to claim 8, wherein a respective valve is associated to each of said at least one reagent and calibrator compartments, to control the delivery thereof.

10. Apparatus according to claim 9, wherein in each compartment the respective reagent or calibrator kept under pressure.

11. Apparatus according to claim 1, wherein said means for interrupting the fluid connection are formed by cut-off valve.

12. Apparatus according to claim 1, wherein said means for determining the concentration of the analytes are constituted of one of a photometric and colorimetric detectors.

13. Apparatus according to claim 1, wherein at least one element of a group comprising of reduction columns, thermostated coils, dialysis units, distillation columns and ionic exchange resins is arranged to cooperate with said means for determining the concentration of analytes.

14. Apparatus according to claim 1, wherein said expansion chamber is fixed volume chamber, capable of maintaining in an inside thereof an overpressure, caused by the action of said bidirectional pump means.

15. Apparatus according to claim 1, wherein said means for discharging an recirculating liquid samples are constituted by a three-way valve.

16. Apparatus according to claim 15, wherein said means for discharging and recirculating liquid samples further comprise a two-way valve situated between said means for sampling and said pump means.

17. Apparatus according to claim 1, wherein said means for sampling and said means for discharging and recirculating liquid samples are arranged in a same unit.