Apparatus for the determination of analytes in liquid samples
First Claim
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1. Apparatus for determining analytes in liquid samples which comprisesa) means for sampling;
- b) bidirectional pump means in fluid connection with said means for sampling;
c) an injection chamber in fluid connection with said pump means, through a bottom input, said injection chamber comprising a first outlet tube which draws to an inside thereof;
d) means for controlling addition of at least one reagent and calibrator into said injection chamber while in fluid connection with said injection chamber;
e) means for determining concentration of said analytes, in fluid connection with said injection chamber through said first outlet tube;
f) means for interrupting the fluid connection between said injection chamber and said means for determining the concentration of analytes;
g) an expansion chamber in fluid connection with said means for determining concentration, through an inlet tube which draws to an inside thereof, said expansion chamber comprising a second outlet tube;
h) means for discharging and recirculating the liquid samples in fluid connection with said expansion chamber through said second outlet tube and with said means for sampling andi) means for operating and controlling the apparatus, wherein said means for controlling addition is responsive to the fluid flow from said injection chamber to said expansion chamber, whereby said apparatus operates as a single hydraulic circuit closed to the external air.
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Abstract
An apparatus for the determination of analytes in liquid samples is disclosed. The apparatus comprises in fluid connection a positive-displacement pump, a chamber in which the reagents specific for the analytes to be determined can be delivered, a detector, a second chamber and a device for the introduction of sample within the circuit and for the discharge. The apparatus also provides for apparatus to control flow and to control of the operation of each component. A process for the determination of the analytes is also disclosed.
135 Citations
17 Claims
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1. Apparatus for determining analytes in liquid samples which comprises
a) means for sampling; -
b) bidirectional pump means in fluid connection with said means for sampling; c) an injection chamber in fluid connection with said pump means, through a bottom input, said injection chamber comprising a first outlet tube which draws to an inside thereof; d) means for controlling addition of at least one reagent and calibrator into said injection chamber while in fluid connection with said injection chamber; e) means for determining concentration of said analytes, in fluid connection with said injection chamber through said first outlet tube; f) means for interrupting the fluid connection between said injection chamber and said means for determining the concentration of analytes; g) an expansion chamber in fluid connection with said means for determining concentration, through an inlet tube which draws to an inside thereof, said expansion chamber comprising a second outlet tube; h) means for discharging and recirculating the liquid samples in fluid connection with said expansion chamber through said second outlet tube and with said means for sampling and i) means for operating and controlling the apparatus, wherein said means for controlling addition is responsive to the fluid flow from said injection chamber to said expansion chamber, whereby said apparatus operates as a single hydraulic circuit closed to the external air. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification