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Method and system for determining bioactive substances

  • US 5,411,866 A
  • Filed: 03/30/1993
  • Issued: 05/02/1995
  • Est. Priority Date: 03/30/1993
  • Status: Expired due to Fees
First Claim
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1. A detection system for measuring glutamine in a liquid sample using enzymatic degradation of glutamine and amperometric detection of the resulting product or element, in the presence of compounds interfering with the measurement, the system comprising in combination:

  • a) an ion exchange means capable of at least partly removing from the sample passed therethrough the interfering compounds while leaving the measured glutamine therein by virtue of a difference in their respective electric charges,b) immobilized enzymes glutaminase and glutamate oxidase for the enzymatic degradation of the measured glutamine, the enzymes being associated with the ion exchange means downstream thereof, andc) a sensor capable of sensing a product or element resulting from the enzymatic degradation of said glutamine to produce a signal indicative of the concentration of glutamine in the sample.

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