Production of hyaluronic acid
First Claim
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1. A process for the production of hyaluronic acid comprising:
- (a) culturing Streptococcus equi by fermentation in a nutrient medium containing;
an assimilable source of carbon;
a source of nitrogen;
sources of phosphorus, sodium, potassium, magnesium, iron, zinc and manganese;
sources of growth factors; and
a source of sulphur,characterized in that the process comprises continuous fermentation of Streptococcus equi in a chemostat culture which is maintained at a pH of from 6.0 to 7.0, a dilution rate of 0.05 to 0.12 h-1, and a dissolved oxygen tension of less than 1% saturation to produce a biomass containing hyaluronic acid, and(b) recovering said hyaluronic acid from said biomass.
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Abstract
A process for the production of hyaluronic acid by continuous fermentation of Streptococcus equi in a chemostat culture gives high yields of high molecular weight hyaluronic acid uncontaminated by toxic impurities. The process is advantageous in that it solves the problem of traditional batch culture in which degradation enzymes can begin to break down the cell walls of Streptococcus releasing cell contents into the fermenter broth, leading to purification difficulties.
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14 Claims
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1. A process for the production of hyaluronic acid comprising:
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(a) culturing Streptococcus equi by fermentation in a nutrient medium containing; an assimilable source of carbon; a source of nitrogen; sources of phosphorus, sodium, potassium, magnesium, iron, zinc and manganese; sources of growth factors; and a source of sulphur, characterized in that the process comprises continuous fermentation of Streptococcus equi in a chemostat culture which is maintained at a pH of from 6.0 to 7.0, a dilution rate of 0.05 to 0.12 h-1, and a dissolved oxygen tension of less than 1% saturation to produce a biomass containing hyaluronic acid, and (b) recovering said hyaluronic acid from said biomass. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification