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Production of hyaluronic acid

  • US 5,411,874 A
  • Filed: 07/06/1993
  • Issued: 05/02/1995
  • Est. Priority Date: 11/07/1990
  • Status: Expired due to Term
First Claim
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1. A process for the production of hyaluronic acid comprising:

  • (a) culturing Streptococcus equi by fermentation in a nutrient medium containing;

    an assimilable source of carbon;

    a source of nitrogen;

    sources of phosphorus, sodium, potassium, magnesium, iron, zinc and manganese;

    sources of growth factors; and

    a source of sulphur,characterized in that the process comprises continuous fermentation of Streptococcus equi in a chemostat culture which is maintained at a pH of from 6.0 to 7.0, a dilution rate of 0.05 to 0.12 h-1, and a dissolved oxygen tension of less than 1% saturation to produce a biomass containing hyaluronic acid, and(b) recovering said hyaluronic acid from said biomass.

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