Dry slide for diagnostic tests

• US 5,411,893 A
• Filed: 03/15/1993
• Issued: 05/02/1995
• Est. Priority Date: 03/15/1993
• Status: Expired due to Term

• Current Assignee: Difco Laboratories, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Difco Laboratories, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Smith, Jerry W., Eden, Ruth F., Strenkoski, Leon F., Meszaros, Amy T.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US08/031,660
• Time in Patent Office: 778 Days
• Field of Search: 422/56, 422/57, 422/58, 422/61, 422/73, 422/82.05, 422/82.08, 422/82.09, 422/86, 422/88, 436/69, 436/165, 436/121, 436/167, 436/110, 436/169, 436/106, 436/805, 436/808, 435/4, 435/7.1, 435/7.2, 435/7.4, 435/12, 435/13, 435/19, 435/21, 435/24, 435/27, 435/285, 435/288, 435/291, 435/808, 435/805, 356/244
• US Class Current: 436/165
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: G01N 33/521 Single-layer analytical elements
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 436/808 Automated or kit

Abstract

A test slide (10) for performing diagnostic tests for detecting the presence of cells, their enzymes, metabolites and other cellular derivatives. The test slide (10) comprises a support strip (14), a coating (34) comprising a carrier and a diagnostic reagent disposed on the support strip (14), and a mount (12) comprising a front wall (18) and a rear wall (20). The coating (34) has the properties of being dry and stable, and water soluble. Further, the coating (34) and the diagnostic reagent are capable of rehydration. The support strip (14) is disposed between the front wall (18) and the rear wall (20) of the mount (12). Both the front (18) and rear (20) walls can have coinciding openings (28,28a) that overlie the support strip (14) thereby allowing spectroscopic, visual and microscopic examination of the support strip (14).

Claims

1. A test slide for performing diagnostic tests for detecting the presence of cells, their enzymes, metabolites and other cellular derivatives, said test slide comprising:

a support strip being dimensionally stable and having a top surface and a bottom surface,

a coating directly adhering to said support strip,

said coating comprising a carrier and a diagnostic reagent,

said coating being dry and stable, and water soluble,

said coating and said reagent being capable of rehydration,

a mount comprising a front wall and a rear wall,

said support strip being disposed between said front wall and said rear wall, and

wherein said front and rear walls having at least one coinciding opening that overlie said support strip thereby allowing spectroscopic, visual and microscopic examination of said support strip.

2. The test slide of claim 1 wherein the front and rear walls have four coinciding openings.

3. The test slide of claim 2 further comprising at least one of said openings formed in said front wall without said coinciding opening in said rear wall.

4. The test slide of claim 1 wherein said coating comprises a plurality of diagnostic reagents.

5. The test slide of claim 1 further comprising said support strip being composed of glass, plastic material or paper material.

6. The test slide of claim 5 further comprising by said support strip being in the form of a plastic film.

7. The support strip of claim 5 wherein said plastic material is selected from the group consisting of polyethylene, polyester, polyvinyl chloride, polyethylene terephthalate, polyethylene terephthalate glycol, cellulose triacetate, and polycarbonate.

8. The support strip of claim 5 wherein said paper material is selected from the group consisting of absorbent paper, plastic-backed paper, membrane covered absorbent paper, and porous membrane covered absorbent paper.

9. The test slide of claim 1 further comprising said coating covering said entire top surface.

10. The test slide of claim 1 wherein said carrier is selected from the group consisting of gelatin, polygalacturonic acid, pectin, agar, agarose, cellulose, carboxymethyl cellulose, guar, xanthan, acacia, plant gums, starch, polyvinyl alcohol, polyvinyl chloride and polyacrylamide.

11. The test slide of claim 1 wherein said front and rear walls include opposing internal surfaces, further comprising by an outer margin of said support strip attached to said mount by an adhesive disposed on either or both opposing internal surfaces at least in an area surrounding said openings.

12. The test slide of claim 11 further comprising said adhesive covering said opposing internal surfaces such that said internal surfaces are bound to one another and thereby said front and rear walls are securely fastened to each other.

13. The test slide of claim 11 further comprising a clasp holding said opposing internal surfaces contiguous such that said front and rear walls are securely fastened with the ability to open said mount.

14. The test slide of claim 1 wherein said front and rear walls include opposing internal surfaces, further comprising a clasp holding said opposing internal surfaces contiguous with said support strip sandwiched between such that said front and rear walls are securely fastened.

15. The test slide of claim 1 wherein said mount is of a semi-rigid material selected from the group consisting of plastic material and cardboard material.

22. The method of claim 6 wherein said step of bonding the coating includes applying the coating to the entire top surface.

31. A method of detecting the presence of cells:

their enzymes, metabolites and other cellular derivatives comprising;

providing the test slide of claim 1, using the test applying a cell, its enzymes, metabolites or other cellular derivatives on the coating on the support strip through the opening adjacent the top surface of the support strip through the opening, and

observing any spectroscopic, visible or microscopic changes which occur indicating the diagnostic reagent has changed in response to the application.

32. A test slide as set forth in claim 1 wherein said coating impregnates said support strip.

16. A method of making a test slide for performing diagnostic tests for detecting the presence of cells, their enzymes, metabolites and other cellular derivatives, said method comprising:

forming a support strip being dimensionally stable and having a top surface and a bottom surface,

preparing a coating comprising a carrier and a diagnostic reagent being dry and stable, water soluble, and capable of rehydration,

bonding the coating by its own properties to the support strip,

mounting the support strip in a mount comprising a front wall and a rear wall, the support strip being disposed between the front wall and the rear wall, and

placing at least one coinciding openings in the front wall and the rear wall that overlie the support strip such that spectroscopic, visual and microscopic examination of changes in the diagnostic reagent disposed on the support strip can be monitored.

17. The method of claim 16 wherein said step of preparing the coating includes a plurality of diagnostic reagents.

18. The method of claim 16 further comprising forming the support strip of glass, plastic material or paper material.

19. The method of claim 18 wherein said step of forming the support strip is in the form of a plastic film.

20. The method of claim 18 further comprising selecting the plastic material from the group consisting of polyethylene, polyester, polyvinyl chloride, polyethylene terephthalate, polyethylene terephthalate glycol, cellulose triacetate, and polycarbonate.

21. The method of claim 18 further comprising selecting paper material from the group consisting of absorbent paper, plastic-backed paper, membrane covered absorbent paper, and porous membrane covered absorbent paper.

23. The method of claim 17 further comprising selecting the carrier from the group consisting of gelatin, polygalacturonic acid, pectin, agar, agarose, cellulose, carboxymethyl cellulose, guar, xanthan, acacia, plant gums, starch, polyvinyl alcohol, polyvinyl chloride and polyacrylamide.

24. The method of claim 16 further comprising forming four coinciding openings in each of the front and rear walls of the mount.

25. The method of claim 24 further comprising forming at least one of said openings in said front wall without said coinciding opening in said rear wall.

26. The method of claim 16 wherein said step of mounting the support strip includes forming front and rear walls having opposing internal surfaces, and an outer margin of the support strip attached to the mount by an adhesive disposed on either or both opposing internal surfaces at least in an area surrounding the openings.

27. The method of claim 26 further comprising covering the opposing internal surfaces with the adhesive such that the internal surfaces are bound to one another and thereby said front and rear walls are securely fastened to each other.

28. The method of claim 26 further comprising holding the opposing internal surfaces contiguous such that said front and rear walls are securely fastened by a clasp.

29. The method of claim 16 further comprising forming front and rear walls having opposing internal surfaces and holding the opposing internal surfaces contiguous with said support strip sandwiched between such that said front and rear walls are securely fastened by a clasp.

30. The method of claim 16 further comprising forming the mount from a semi-rigid material selected from the group consisting of plastic material and cardboard material.

33. A method as set forth in claim 16 wherein said step of bonding the coating incudes spot dispensing the coating onto the top surface.

34. A method as set forth in claim 16 wherein said step of bonding the coating includes impregnating the support strip with the coating.