Carbohydrate heterobifunctional cross-linking reagent
First Claim
Patent Images
1. A carbohydrate cross-linking reagent comprising a sequence of substituted glucosamine units in β
- -1,4 linkage, said sequence having at the reducing end a tetrahydrofuran unit, said reagent having the formula ##STR20## wherein R1, R2 is H, (CH2 CH2 O)m H, (CH2 CH [CH3 ]O)m H, CH2 CO2 H, or CH2 CH2 CN, wherein m is an integer in the range of 1-10;
n is greater than or equal to 2;
R3 and R4 are acylating, alkylating, electrophilic or nucleophilic reactive substituents which are different from each other but mutually compatible neither R3 nor R4 is an acyl group, and at least one R4 is present; and
R5 is an acyl group.
1 Assignment
0 Petitions
Accused Products
Abstract
A heterobifunctional cross-linking reagent having a carbohydrate backbone (derived from a chitin substrate) is disclosed. The cross-linker is available in a variety of lengths, is highly water-soluble, and can be formulated to undergo cleavage catalyzed by the enzyme lysozyme.
42 Citations
17 Claims
-
1. A carbohydrate cross-linking reagent comprising a sequence of substituted glucosamine units in β
- -1,4 linkage, said sequence having at the reducing end a tetrahydrofuran unit, said reagent having the formula ##STR20## wherein R1, R2 is H, (CH2 CH2 O)m H, (CH2 CH [CH3 ]O)m H, CH2 CO2 H, or CH2 CH2 CN, wherein m is an integer in the range of 1-10;
n is greater than or equal to 2; R3 and R4 are acylating, alkylating, electrophilic or nucleophilic reactive substituents which are different from each other but mutually compatible neither R3 nor R4 is an acyl group, and at least one R4 is present; and R5 is an acyl group. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
- -1,4 linkage, said sequence having at the reducing end a tetrahydrofuran unit, said reagent having the formula ##STR20## wherein R1, R2 is H, (CH2 CH2 O)m H, (CH2 CH [CH3 ]O)m H, CH2 CO2 H, or CH2 CH2 CN, wherein m is an integer in the range of 1-10;
-
2. A carbohydrate cross-linking reagent comprising a sequence of substituted glucosamine units in β
- -1,4 linkage, said sequence having at the reducing end a tetrahydrofuran unit, said reagent having the formula ##STR21## wherein R1, R2 is H, (CH2 CH2 O)m H, (CH2 CH [CH3 ]O)m H, CH2 CO2 H, or CH2 CH2 CN, wherein m is an integer in the range of 1-10;
n is greater than or equal to 1; R3 and R4 are acylating, alkylating, electrophilic or nucleophilic reactive substituents which are different from each other but mutually compatible neither R3 nor R4 is an acyl group; and R5 is an acyl group.
- -1,4 linkage, said sequence having at the reducing end a tetrahydrofuran unit, said reagent having the formula ##STR21## wherein R1, R2 is H, (CH2 CH2 O)m H, (CH2 CH [CH3 ]O)m H, CH2 CO2 H, or CH2 CH2 CN, wherein m is an integer in the range of 1-10;
Specification