Pollen-specific promoter from maize

• US 5,412,085 A
• Filed: 11/09/1993
• Issued: 05/02/1995
• Est. Priority Date: 07/09/1992
• Status: Expired due to Term

• Current Assignee: Pioneer Hi-Bred International (Corteva, Inc.)
• Original Assignee: Pioneer Hi-Bred International (Corteva, Inc.)
• Inventors: Allen, Rebecca L., Lonsdale, David M.
• Primary Examiner(s): Fox, David T.
• Assistant Examiner(s): McElwain, Elizabeth F.
• Application Number:
  US08/149,695
• Time in Patent Office: 539 Days
• Field of Search: 800/205, 800/DIG. 56, 435/172.3, 435/320.1, 935/41, 935/6, 935/67, 536/24.1
• US Class Current: 536/24.1
• CPC Class Codes: C12N 15/8222 Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
• CPC Class Codes: C12N 15/8231 Male-specific, e.g. anther, tapetum, pollen
• CPC Class Codes: C12N 9/2402 hydrolysing O- and S- glycosyl compounds (3.2.1)
• CPC Class Codes: C12Y 302/01015 Polygalacturonase (3.2.1.15)

Abstract

The present invention is related to isolated purified DNA sequences isolated from maize which can act as pollen-specific promoters and which play a role in the expression of genes in pollen. The present invention also relates to a method for conferring pollen-specificity on genes not normally expressed in pollen.

Claims

1. An isolated and purified DNA molecule consisting essentially of a nucleotide sequence which is selected from the group consisting of SEQ ID NO:

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7 and SEQ ID NO;

8.

3. A pollen-specific chimeric gene comprising:

(a) the DNA molecule as in either claim 1 (SEQ ID NO;

2-8) or claim 2 (SEQ ID NO;

     5); and

(b) an exogenous gene,

wherein said DNA molecule regulates the expression of said exogenous gene in a pollen-specific manner.

4. A transfer vector comprising the pollen-specific chimeric gene of claim 3.

2. An isolated and purified DNA molecule consisting essentially of the nucleotide sequence of SEQ ID NO:

5.

5. An isolated and purified DNA molecule that consists of a nucleotide sequence which is selected from the group consisting of SEQ ID NO:

2, SEQ ID NO;

3, SEQ ID NO;

4, SEQ ID NO;

5, SEQ ID NO;

6, SEQ ID NO;

7 and SEQ ID NO;

8.

7. A pollen-specific chimeric gene comprising:

(a) the DNA molecule of claim 5; and

(b) an exogenous gene,

wherein said DNA molecule regulates the expression of said exogenous gene in a pollen-specific manner.

8. A transfer vector comprising the pollen-specific chimeric gene of claim 7.

6. An isolated and purified DNA molecule consisting of the nucleotide sequence of SEQ ID NO:

5.

9. A pollen-specific chimeric gene comprising:

(a) the DNA molecule of claim 6 (SEQ ID NO;

     5); and

(b) an exogenous gene,

wherein said DNA molecule regulates the expression of said exogenous gene in a pollen-specific manner.

10. A transfer vector comprising the pollen-specific chimeric gene of claim 9.