Process for producing plantlets whereby the formation of a cell mass is induced

• US 5,413,929 A
• Filed: 05/28/1991
• Issued: 05/09/1995
• Est. Priority Date: 09/30/1989
• Status: Expired due to Term

• Current Assignee: Kirin Beer Kabushiki Kaisha
• Original Assignee: Kirin Beer Kabushiki Kaisha
• Inventors: Onishi, Noboru, Ishizaki, Keiichirou
• Primary Examiner(s): Robinson, Douglas W.
• Assistant Examiner(s): DADIO, SUSAN
• Application Number:
  US07/689,949
• Time in Patent Office: 1,442 Days
• Field of Search: 435/240.4, 435/240.45, 435/240.46, 435/240.51, 435/240.54
• US Class Current: 435/430
• CPC Class Codes: A01H 4/005 Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques

Abstract

The present invention relates to a process for producing plantlets using plant tissue culture techniques. The object of the invention is to provide a process which enables simple, highly reproducible and efficient production of seedlings in a large scale. The feature of the invention comprises culturing a root as an explant in a liquid medium containing at least inorganic salts, a carbon source and an auxin to induce the formation of a cell mass, and culturing the resultant cell mass in a redifferentiation medium containing at least inorganic salts and a carbon source, to produce plantlets.

Claims

1. A process for producing a plantlet which comprises culturing a root as an explant in a liquid induction medium comprising inorganic salts, a carbon source and an auxin, wherein the concentration of said auxin is in a range from 0.1 to 5 mg./liter, to induce the formation of a cell mass, and culturing the resultant cell mass in a redifferentiation medium, comprising inorganic salts and a carbon source, to produce a plantlet.

3. The process of claim 1 or 2, wherein said root is derived from a plant belonging to the family Liliaceae or the family Umbelliferae.

4. The process of claim 1 or 2 further comprising agitating the medium.

5. The process of claim 4 wherein said agitating is performed by stirring.

6. The process of claim 4 wherein said agitating is performed by rotation.

7. The process of claim 4 wherein said agitating is performed by shaking.

2. A process for producing a plantlet which comprises culturing a root as an explant in a liquid induction medium comprising inorganic salts, a carbon source and an auxin, wherein the concentration of said auxin is in a range from 0.1 to 5 mg./liter, to induce the formation of a cell mass, then further culturing said cell mass in a liquid propagation medium comprising inorganic salts and a carbon source for further propagation, and subsequently culturing the resultant cell mass in a redifferentiation medium comprising inorganic salts and a carbon source, to produce a plantlet.