Apparatus and method for amplifying and detecting target nucleic acids
First Claim
1. An apparatus for thermal cycling a sample of nucleic acid contained in an elongated reaction vessel defining a longitudinal axis, the apparatus comprising:
- a first heat conducting holder having at least one aperture means for receiving a first segment of an elongated reaction vessel, said first holder including first heating element means for providing heat to said first segment; and
a second heat conducting holder having at least one aperture means, said at least one aperture means in registration with said at least one aperture means of said first holder for receiving a second segment of said elongated reaction vessel, said second holder including second heating element means for providing heat to said second segment independently of heat provided to the first segment by said first heating element;
wherein said first segment is longitudinally spaced from said second segment.
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Accused Products
Abstract
Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction detection unit. A first heating element of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target.
412 Citations
14 Claims
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1. An apparatus for thermal cycling a sample of nucleic acid contained in an elongated reaction vessel defining a longitudinal axis, the apparatus comprising:
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a first heat conducting holder having at least one aperture means for receiving a first segment of an elongated reaction vessel, said first holder including first heating element means for providing heat to said first segment; and a second heat conducting holder having at least one aperture means, said at least one aperture means in registration with said at least one aperture means of said first holder for receiving a second segment of said elongated reaction vessel, said second holder including second heating element means for providing heat to said second segment independently of heat provided to the first segment by said first heating element; wherein said first segment is longitudinally spaced from said second segment. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification