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Analyte immunoassay in self-contained apparatus

  • US 5,416,000 A
  • Filed: 11/21/1991
  • Issued: 05/16/1995
  • Est. Priority Date: 03/16/1989
  • Status: Expired due to Term
First Claim
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1. A method for detecting the presence of an analyte in a sample, wherein said analyte is a member of a specific binding pair, employing a signal producing system having a reagent bound non-diffusibly to a surface and at least one reagent free in solution, said signal producing system with said analyte producing a detectable signal in a signal measurement region on a measuring strip as a said surface, wherein a complementary member of said signal producing system specific for said analyte is said reagent non-diffusibly bound to said surface and a solution comprising a labelled detecting member of said signal producing system is provided, wherein said labelled detecting member binds to said analyte or said complementary member bound non-diffusibly to said surface, said method comprising:

  • applying said sample to a sample receiving element;

    bringing said sample receiving element into fluid transfer relationship with a transfer strip and said measuring strip;

    flowing transport solution through said transfer strip, sample receiving element and measurement region in the direction of flow, whereby any analyte in said sample is transported from said sample receiving element to said measurement region in conjunction with said labelled detecting member, wherein said labelled detecting member may be competitive with said analyte for said non-diffusibly bound complementary member or complementary to said analyte; and

    determining the distance said labelled detecting member bound to said surface has migrated in said measurement region as a measure of the amount of analyte in the sample.

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