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Multilayer analytical element containing primary amine buffer and method for the determination of ethanol

  • US 5,416,004 A
  • Filed: 01/19/1993
  • Issued: 05/16/1995
  • Est. Priority Date: 01/19/1993
  • Status: Expired due to Term
First Claim
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1. An analytical element for the determination of ethanol comprising a support having thereon, in order and in fluid contact:

  • a) a first reagent layer containing a buffer having a primary amine mixed with an uncrosslinked hydrophilic binder, said buffer maintaining the pH at from about 8 to about 10 during an assay for ethanol and being present in an amount of at least about 25 mmoles/m2,b) a second reagent layer comprising a crosslinked hydrophilic binder,c) a third reagent layer comprising alcohol dehydrogenase and a buffer present in an amount of at least about 25 mmoles/m2 mixed with an uncrosslinked hydrophilic binder, said buffer being the same as that in said first reagent layer and maintaining the pH at from about 8 to about 10, andd) a porous spreading layer containing an oxidized nicotinamide coenzyme.

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