Method for detecting the change in an analyte due to hemolysis in a fluid sample
First Claim
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1. A method of estimating a change in an analyte in a whole-blood sample which is due to the hemolysis of red blood cells, comprising:
- (a) contacting a whole-blood sample comprising intact red blood cells with a dry separation material having a site where said whole blood sample is introduced and a remainder, said material having physical characteristics effective to separate by wicking action a fraction, which contains extracellular hemoglobin that may be present in said sample, from the intact red blood cells;
(b) allowing said sample to remain in contact with said material for a period of time sufficient to effect said separation;
(c) detecting at a location in said remainder the presence of extracellular hemoglobin in said fraction; and
(d) estimating the change in said analyte in said sample which is due to the hemolysis of red blood cells.
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Abstract
The present invention concerns a method of detecting hemolysis in a whole-blood sample, a method of determining an elevation in the potassium ion concentration of a whole-blood sample, an apparatus for detecting hemolysis and/or determining an elevation in the potassium ion concentration in a fluid sample, an apparatus for detecting hemolysis and/or determining an elevation in the potassium ion concentration in a whole-blood sample, and a single-use cartridge containing a plurality of microfabricated biosensors which further contains a hemolysis detection unit.
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Citations
27 Claims
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1. A method of estimating a change in an analyte in a whole-blood sample which is due to the hemolysis of red blood cells, comprising:
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(a) contacting a whole-blood sample comprising intact red blood cells with a dry separation material having a site where said whole blood sample is introduced and a remainder, said material having physical characteristics effective to separate by wicking action a fraction, which contains extracellular hemoglobin that may be present in said sample, from the intact red blood cells; (b) allowing said sample to remain in contact with said material for a period of time sufficient to effect said separation; (c) detecting at a location in said remainder the presence of extracellular hemoglobin in said fraction; and (d) estimating the change in said analyte in said sample which is due to the hemolysis of red blood cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 23, 24, 25, 26, 27)
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21. A method of determining the elevation in the potassium ion concentration due to hemolysis of intact red blood cells in a whole-blood sample, comprising:
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(a) contacting a whole-blood sample comprising intact red blood cells with a dry separation material having a site where said whole blood sample is introduced and a remainder, said material having physical characteristics effective to separate by wicking action a plasma fraction, which contains extracellular hemoglobin that may be present in said sample, from the intact red blood cells; (b) allowing said sample to remain in contact with said material for a period of time sufficient to effect said separation in said remainder; (c) estimating the quantity of extracellular hemoglobin in said plasma fraction; and (d) estimating the elevation of the potassium ion concentration in said sample which is due to hemolysis.
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Specification