Method and apparatus for localization and spectroscopy of objects using optical frequency modulation of diffusion waves

• US 5,416,582 A
• Filed: 02/11/1993
• Issued: 05/16/1995
• Est. Priority Date: 02/11/1993
• Status: Expired due to Term

• Current Assignee: The United States of America As Represented By The Secretary of Agriculture
• Original Assignee: United States Department Of Health And Human Services
• Inventors: Schmitt, Joseph M., Knutson, Jay R., Knuttel, Alexander
• Primary Examiner(s): Hille, Rolf
• Assistant Examiner(s): TRAN, MINH LOAN
• Application Number:
  US08/015,983
• Time in Patent Office: 824 Days
• Field of Search: 356/349, 356/345, 128/633, 128/665, 128/653.1
• US Class Current: 356/484
• CPC Class Codes: G01N 21/4795 spatially resolved investigating of object in scattering medium in vivo A61B

Abstract

An apparatus and method for using frequency modulated diffusive photon-density waves propagating through a turbid medium such as tissue for detecting optical properties of objects and for imaging objects within the turbid medium. Frequency modulation enables real time differential measurement of the interaction of different colors with the object when the different colors are chosen such that they react the same with the medium but differently with the object. The method greatly simplifies optical imaging and increases the amount of information which may be obtained about the object.

Claims

1. A method for determining an optical property of an object located in a turbid medium comprising the steps of:

generating a beam of amplitude modulated electromagnetic radiation which alternates between different colors at a predetermined modulation rate;

choosing said different colors such that said different colors interact with said object differently while interaction between said different colors and said turbid medium is substantially the same;

applying said beam to said medium to generate diffuse photon density waves within said medium such that said diffuse photon density waves interact with said object;

detecting output from said medium and storing a differential signal resulting from said modulation at said predetermined modulation rate; and

deriving information about said optical property of said object from said differential signal.

2. A method as recited in claim 1 wherein said choosing step comprises choosing said different colors such that one of said different colors is absorbed by said object and another of said different colors is not absorbed by said object.

3. A method as recited in claim 1 further comprising the step of leveling optical power of said beam so that optical power associated with each of said different colors is substantially equal.

4. A method as recited in claim 1 further comprising the steps of:

splitting said beam into at least two beams; and

applying multiple beams to said medium.

5. A method as recited in claim 4 further comprising the step of generating a phase difference between at least two of said multiple beams so as to produce one of constructive and destructive interference of said diffuse photon density waves.

6. A method as recited in claim 1 further comprising the step of scanning said beam across a portion of said medium.

7. A method as recited in claim 1 further comprising the step of spatially localizing said object utilizing said information about said optical property.

8. A method as recited in claim 1 wherein said generating step further comprises the step of generating said electromagnetic radiation such that the wavelength of said electromagnetic radiation is in the range between 100 nm to 1 mm.

9. A method as recited in claim 1 wherein said generating step further comprises the steps of:

generating individual pulses from at least two different lasers, said different lasers corresponding to said different colors;

optically combining output of said individual lasers to form a single beam path; and

timing said individual pulses in accordance with said predetermined modulation rate to generate said beam alternating between said different colors.

10. An apparatus for determining an optical property of an object located in a turbid medium comprising:

a source of amplitude modulated electromagnetic radiation which alternates between different colors at a predetermined modulation rate, said different colors selected such that said object interacts with said different colors differently and said medium interacts with said different colors substantially the same;

means for applying said beam to said medium to generate diffuse photon density waves within said medium such that said diffuse photon density waves interact with said object;

means for receiving the output from said medium and storing a differential signal resulting from said modulation at said predetermined modulation rate; and

means for deriving information about said optical property of said object from said differential signal.

11. A apparatus as recited in claim 10, wherein one of said different colors is absorbed by said object and another of said different colors is not absorbed by said object.

12. A apparatus as recited in claim 10, further comprising means for leveling optical power of said beam so that optical power associated with each of said different colors is substantially equal.

13. A apparatus as recited in claim 10, further comprising:

means for splitting said beam into at least two beams; and

means for directing multiple beams to said medium.

14. A apparatus as recited in claim 13, further comprising the step of generating a phase difference between at least two of said multiple beams so as to produce one of constructive and destructive interference of said diffuse photon density waves.

15. A apparatus as recited in claim 10, further comprising means for scanning said beam across a portion of said medium.

16. A apparatus as recited in claim 10, further comprising means for spatially localizing said object utilizing said information about said optical property.

17. A apparatus as recited in claim 10, wherein said electromagnetic radiation comprises Wavelengths in the range between 100 nm to 1 mm.

18. A apparatus as recited in claim 10, wherein said source comprises:

at least two different lasers, said different lasers corresponding to said different colors;

means for optically combining output of said individual lasers to form a single beam path; and

means for alternating said individual pulses in accordance with said predetermined modulation rate to generate said beam alternating between said different colors.

19. A method for determining an optical property of an object located in a-turbid medium comprising the steps of:

generating a beam of amplitude modulated electromagnetic radiation which is frequency modulated to alternate between different colors at a predetermined modulation frequency;

choosing said different colors such that said different colors interact with said object differently while interaction between said different colors and said turbid medium is substantially the same;

applying said beam to said medium to generate diffuse photon density waves within said medium such that said diffuse photon density waves interact with said object;

detecting output from said medium and generating a signal corresponding to said output;

processing said signal such that phase and amplitude information arising from modulations in photon density from both said amplitude modulation frequency and said frequency modulation frequency is retained; and

deriving information about a location of said object and said optical property of said object by analyzing a spatial pattern and color variation in said phase and amplitude information.

20. A method as recited in claim 19, wherein said processing step further comprises the steps of:

heterodyne mixing said signal from a photodetector with a predetermined frequency to produce a mixed signal;

filtering said mixed signal to produce a filtered signal;

convert said filtered signal to a digital signal to facilitate processing; and

isolating components of said signal at frequencies corresponding to combinations of said amplitude modulated frequency, said frequency modulation frequency and said predetermined frequency.

21. An apparatus for determining a property of an object located in a medium, comprising:

a source of amplitude modulated electromagnetic radiation which is frequency modulated between different frequencies at a predetermined modulation rate, said different frequencies being selected such that said object interacts with said different frequencies differently and said medium interacts with said different frequencies substantially the same, said source of electromagnetic radiation being incident upon said medium;

a detector located to detect at least a portion of said electromagnetic radiation after said portion has interacted with said object and said medium, said detector producing an output signal representative of said detected electromagnetic radiation; and

a processor coupled to receive said output signal, said processor determining said property of said object from said output signal.