Test articles for performing dry reagent prothrombin time assays
DCFirst Claim
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1. A test article for performing dry reagent prothrombin time assays, said test article comprisinga solid phase matrix;
- dry thromboplastin immobilized on or within the solid phase matrix, wherein the thromboplastin is substantially free from substances found in thromboplastin purified from brain extract which cause aberrant functioning intermediate transition states as the thromboplastin is rehydrated with liquid sample; and
coagulation neutral agents which facilitate rehydration of the thromboplastin upon contact of the solid phase matrix with the liquid sample.
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Abstract
Test articles comprise a solid phase membrane having dry thromboplastin immobilized thereon or within. The thromboplastin is substantially free from substances which might cause aberrant functioning intermediate transition states as the thromboplastin is rehydrated with liquid sample. Coagulation neutral agents which facilitate rehydration of the dry thromboplastin are also provided on the solid phase membrane.
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Citations
9 Claims
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1. A test article for performing dry reagent prothrombin time assays, said test article comprising
a solid phase matrix; -
dry thromboplastin immobilized on or within the solid phase matrix, wherein the thromboplastin is substantially free from substances found in thromboplastin purified from brain extract which cause aberrant functioning intermediate transition states as the thromboplastin is rehydrated with liquid sample; and coagulation neutral agents which facilitate rehydration of the thromboplastin upon contact of the solid phase matrix with the liquid sample. - View Dependent Claims (2, 3)
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4. A test strip comprising:
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a permeable membrane having an application face and an indicator face in lateral opposition, said membrane being substantially free from an interference with a thromboplastin-initiated and factor VII or VIIa mediated coagulation pathway;
coagulation neutral agents which facilitate liquid sample uptake and distribution into the membrane;dry thromboplastin impregnated within the membrane, wherein the thromboplastin is substantially free from substances found in thromboplastin purified from brain extract which cause aberrant intermediate transition states as the thromboplastin is rehydrated with liquid sample; and a substrate impregnated within the membrane, which substrate produces a detectable signal upon activation by a component of the coagulation pathway; whereby coagulation factor VII or VII(a) containing samples may be applied to the application face of the membrane in order to produce the detectable signal on the indicator face as a result of activation of the substrate by the coagulation pathway component. - View Dependent Claims (5, 6)
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- 7. An improved prothrombin time assay of the type wherein a blood or plasma sample is applied to a solid phase matrix to contact dry thromboplastin to initiate a detectable reaction, wherein the improvement comprises providing a coagulation neutral agent within the matrix and contacting a dry thromboplastin which is substantially free from substances found in thromboplastin purified from brain extract which cause aberrant functioning transition states as the thromboplastin is rehydrated with the sample.
Specification