Isolated Renilla luciferase and method of use thereof
First Claim
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1. An isolated genetically engineered polypeptide encoded by an isolated DNA or RNA molecule comprising a nucleotide sequence encoding a Renilla luciferase, wherein said polypeptide has the light-producing activity of Renilla luciferase, and wherein said polypeptide is non-glycosylated.
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Abstract
Genetic material encoding luciferase from the marine coelenterate Renilla has been isolated and characterized. This genetic material allows the production of peptides for use as labels in bioluminescence assays or can itself be directly used to identify luciferase genes from related organisms.
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1 Claim
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1. An isolated genetically engineered polypeptide encoded by an isolated DNA or RNA molecule comprising a nucleotide sequence encoding a Renilla luciferase, wherein said polypeptide has the light-producing activity of Renilla luciferase, and wherein said polypeptide is non-glycosylated.
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Current AssigneeThe University of Georgia Research Foundation, Inc.
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Original AssigneeThe University of Georgia Research Foundation, Inc.
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InventorsLorenz, William W., Cormier, Milton J.
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Primary Examiner(s)Wax, Robert A.
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Assistant Examiner(s)Moore, William W.
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Application NumberUS08/167,650Time in Patent Office525 DaysField of Search435/189, 435/69.1, 435/190, 435/252.3, 435/252.33, 435/320.1, 530/350, 530/300US Class Current435/189CPC Class CodesC12N 9/0069 acting on single donors wit...C12Y 113/12005 Renilla-luciferin 2-monooxy...
