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Method for detecting a target analyte in a sample

  • US 5,418,171 A
  • Filed: 11/30/1992
  • Issued: 05/23/1995
  • Est. Priority Date: 03/28/1991
  • Status: Expired due to Fees
First Claim
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1. A method for detecting the presence or absence of a target analyte in a sample by using a first substance and a second substance both of which are capable of specifically binding to the target analyte, comprising the steps of:

  • forming a mixture by mixing a liquid sample containing the sample and a solution containing the first substance, wherein the first substance is labelled,providing an apparatus comprising(a) a container capable of accommodating said mixture, the container having a transparent portion,(b) an insertion member capable of being inserted into and integrally coupled with the container comprising;

    (i) a supporting member with a barrel portion that fits into said container, said barrel portion containing a porous member which has two surfaces, a first surface that contacts the mixture in said container and a second surface opposite said first surface, wherein the second substance is immobilized on the first surface of the porous member; and

    [(ii)]an absorbent contacting the second surface of the porous member; and

    (ii) a vent hole located either in said barrel portion or in said supporting member above the absorbent;

    pouring the mixture into said container,coupling said container and said insertion member of said apparatus by inserting the insertion member into the container, andwithout washing said porous membrane, detecting an occurrence of a reaction between the target analyte and the first and second substances through the signal emitted from the labelled first substance by observing the main surface of the porous member through the transparent portion of the container.

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