Method for laser induced fluorescence of tissue

• US 5,419,323 A
• Filed: 08/29/1991
• Issued: 05/30/1995
• Est. Priority Date: 12/21/1988
• Status: Expired due to Term

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Rava, Richard P., Mehta, Anand, Kittrell, Carter, An, Kyungwon, Park, Young D., Tong, Lucene, Richards-Kortum, Rebecca, Dasari, Ramachandra R., Cothren, Robert M., Feld, Michael S., Baraga, Joseph J., Taroni, Paola
• Primary Examiner(s): Sykes, Angela D.
• Application Number:
  US07/720,465
• Time in Patent Office: 1,370 Days
• Field of Search: 128/633-634, 128/664-666, 128/653.1, 606/3, 606/14-16
• US Class Current: 600/476
• CPC Class Codes: A61B 18/245 for removing obstructions in blood vessels or calculi
• CPC Class Codes: A61B 2562/0238 Optical sensor arrangements for performing transmission measurements on body tissue
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence

Abstract

A method for laser induced fluorescence of tissue in which laser radiation is used to illuminate and induce fluorescence in the tissue under study to determine the chemical composition or pathologic condition of tissue. The laser radiation and the retrieved fluorescing radiation can be conveyed through a catheter using an array of optical fiber. The fluorescence spectrum of the tissue can be displayed and analyzed to obtain information regarding the chemical composition and medical condition of the tissue inside the human body.

Claims

1. A method of irradiating chromophores in endogenous tissue to identify tissue composition comprising:

selecting a known excitation wavelength of the chromophore within a range of wavelengths at which the chromophore undergoes fluorescence;

irradiating the endogeneous tissue with radiation having at least the selected excitation wavelength within the range;

detecting a fluorescence emission of the tissue resulting from the irradiation thereof;

analyzing the detected emission to determine the presence of the chromophore in the tissue;

forming an attenuation spectrum for a tissue component; and

modulating the detected emission with the attenuation spectrum.

2. The method of irradiating chromophores of claim 1 wherein the step of forming the attenuation spectrum comprises detecting absorption and scattering components of light incident upon the tissue.

3. The method of irradiating chromophores of claim 1 further comprising the steps of:

selecting a second known excitation wavelength of a second chromophore at which the second chromophore undergoes fluorescence;

irradiating simultaneously the endogeneous tissue with radiation having the second wavelength;

detecting a second fluorescence emission of the tissue resulting from the irradiation within the second wavelength; and

analyzing the second detected fluorescence emission to determine the presence of the second chromophore in the tissue.

4. The method of claim 3 further comprising illuminating the tissue at additional selected wavelengths to induce fluorescence of additional chromophores and determining additional pathological characteristics of the tissue.

5. The method of irradiating chromophores of claim 3 further comprising the step of determining the wavelength of peak fluorescence of the first and second chromophores within the first and second emissions.

6. The method of irradiating chromophores of claim 5 further comprising the step of determining the ratio of the two fluorescence peaks.

7. The method of irradiating chromophores of claim 1 further comprising the step of determining the wavelength of peak fluorescence of the chromophore within the emission and determining the ratio of the peak with a selected valley within the emission.

8. The method of irradiating chromophores of claim 1 further comprising determining the concentration of the chromophore within the tissue.

9. The method of claim 1 further comprising:

inserting a catheter containing a multiplicity of optical fibers into an artery in the body and positioning a distal end of said catheter adjacent to tissue to be diagnosed; and

coupling a light source to at least one of said fibers to direct light out of a distal end of the optical fiber or fibers to which the light source is coupled.

10. The method of irradiating chromophores of claim 1 wherein the chromophore comprises tryptophan in arterial tissue.

11. The method of irradiating chromophores of claim 1 wherein the chromophore comprises collagen.

12. The method of irradiating chromophores of claim 1 wherein the chromophore comprises elastin.

13. A method of irradiating chromophores in endogenous tissue to identify tissue composition comprising:

selecting a known excitation wavelength of the chromophore within a range of wavelengths at which the chromophore undergoes fluorescence;

irradiating the endogeneous tissue with radiation having at least the selected excitation wavelength within the range;

detecting a fluorescence emission of the tissue resulting from the irradiation thereof;

analyzing the detected emission to determine the presence of the chromophore in the tissue, including separating a component of the emission resulting from reabsorption by the tissue.

14. The method of irradiating chromophores of claim 13 further comprising forming an attenuation spectrum for a tissue component;

and modulating the detected emission with the attenuation spectrum.

15. The method of irradiation chromophores of claim 14 wherein the step of forming the attenuation spectrum comprises detecting absorption and scattering components of light incident upon the tissue.

16. The method of irradiating chromophores of claim 13 further comprising the steps of:

selecting a second known excitation wavelength of a second chromophore at which the second chromophore undergoes fluorescence;

irradiating simultaneously the endogeneous tissue with radiation having the second wavelength;

detecting a second fluorescence emission of the tissue resulting from the irradiation within the second wavelength; and

analyzing the second detected emission to determine the presence of the second chromophore in the tissue.

17. A method of irradiating chromophores in endogenous tissue to identify tissue composition comprising:

selecting a known excitation wavelength of the chromophore within a range of wavelengths at which the chromophore undergoes fluorescence;

inserting a fiber optic probe into the body of a patient;

irradiating the endogeneous tissue with the fiber optic probe using radiation having at least the selected excitation wavelength within the range;

detecting a fluorescence emission of the tissue resulting from the irradiation thereof;

analyzing the detected emission to determine the presence of the chromophore in the tissue; and

determining the concentration of the chromophore within the tissue.