One-step tray test for release of soluble mediators and apparatus therefore

• US 5,422,270 A
• Filed: 05/19/1987
• Issued: 06/06/1995
• Est. Priority Date: 05/19/1987
• Status: Expired due to Term

• Current Assignee: United States Department Of Health And Human Services
• Original Assignee: United States Department Of Health And Human Services
• Inventors: Caspi, Rachel R.
• Primary Examiner(s): Low, Christopher S. F.
• Application Number:
  US07/051,313
• Time in Patent Office: 2,940 Days
• Field of Search: 435/300, 435/287, 435/240.1, 435/284, 436/809, 422/99, 356/244, 424/9
• US Class Current: 435/288.5
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/16 Microfluidic devices; Capillary tubes integrated microfluidic structures B01L3/5027; microreactors B01J19/0093
• CPC Class Codes: C12M 41/46 of cellular or enzymatic activity or functionality, e.g. cell viability
• CPC Class Codes: Y10S 435/819 Fermentation vessels in series
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte

Abstract

A microculture tray comprising a plurality of pairs of wells, each pair of wells connected by a trough which extends downwardly approximately 2/3 of the depth of the well so that the wells in the pair are connected so as to allow free passage of supernatant but not of cells between the wells.

The tray can be used for rapid testing of animal cells or non-motile microorganisms for the release of soluble mediators.

Claims

1. A microculture tray for the rapid detection of migration from a first well onto a second well of a soluble or non-soluble in situ-produced mediator, comprising

a base plate having a top and a bottom portions;

at least one pair of separate wells having a preset depth consisting of a first and a second well extending downwardly from the top portion to the bottom portion of the base plate, the separate wells being separated from one another by interwell spaces; and

at least one means for allowing free passage of supernatant but not of cells settled in the bottom portion of the wells between first well and the second well, said means connecting the separate wells of the pair of wells, said means extending downwardly from the top of the base plate about 2/3 of the depth of the connected wells.

2. A microculture tray for the rapid detection of migration from a first well onto a second well of a soluble or non-soluble in situ-produced mediator, comprising

a base plate having a top and a bottom portions;

at least one pair of separate wells having a preset depth consisting of a first and a second well extending downwardly from the top portion to the bottom portion of the base plate, the separate wells being separated from one another by interwell spaces; and

at least one trough connecting the separate wells of the pair of wells, the trough extending downwardly from the top of the base plate about 2/3 of the depth of the connected wells to allow free passage of supernatant but not of cells settled in the bottom portion of the wells between the first well, the trough and the second well.

3. The tray of claim 2, wherein the tray is made of a hydrophobic material.

4. The tray of claim 2, wherein the interwell spaces are filled with paraffin.

5. The microculture tray of claim 2 comprising a plurality of pairs of wells.

6. The microculture tray of claim 5 being a 96-well microculture tray.