Nucleic acid material amplification and detection without washing
First Claim
1. In a device for amplidying and detecting nucleic acid material in a closed container by using at least one target strand as a template, said device comprising a reaction compartment containing reagents for amplifying a sample of nucleic acid material, a detection site for detecting amplified nucleic acid material, and storage compartments containing a label and signaling material effective to generate, in combination, a detectable signal, and passageways for fluidly connecting said compartments with said site but closed to the environment,the improvement wherein said device is free of any wash compartment containing a wash liquid substantially free of capture, label, and signal-forming reagents used in storage or reaction compartments,so that no wash stem is used in a sequence of steps comprising the emptying and moving of the contents of said compartments to said detection site.
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Abstract
A device and method are disclosed for amplifying and detecting nucleic acid material. The device and method use a label and signalling material responsive to the label to produce a detectable signal. A surprising result of the method and device is that at least one of the wash steps heretofore required has been eliminated without substantially adversely affecting the results.
426 Citations
3 Claims
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1. In a device for amplidying and detecting nucleic acid material in a closed container by using at least one target strand as a template, said device comprising a reaction compartment containing reagents for amplifying a sample of nucleic acid material, a detection site for detecting amplified nucleic acid material, and storage compartments containing a label and signaling material effective to generate, in combination, a detectable signal, and passageways for fluidly connecting said compartments with said site but closed to the environment,
the improvement wherein said device is free of any wash compartment containing a wash liquid substantially free of capture, label, and signal-forming reagents used in storage or reaction compartments, so that no wash stem is used in a sequence of steps comprising the emptying and moving of the contents of said compartments to said detection site.
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3. A device as defined in claims 1or wherein said label is an enzyme.
Specification