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Method of performing affinity separation using immobilized flocculating agent on chromatographic support

  • US 5,422,284 A
  • Filed: 10/29/1993
  • Issued: 06/06/1995
  • Est. Priority Date: 07/16/1987
  • Status: Expired due to Fees
First Claim
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1. A method of performing an affinity separation comprising the steps of;

  • a. forming a primary chromatographic support with a flocculating agent immobilized thereon, the primary support selected from the group consisting of particulate resins and porous plastic materials;

    b. forming a secondary support consisting of microparticles having ligand or binder for the ligand affixed thereon;

    c. contacting the primary support of step a with the secondary support of step b to form an affinity support with said microparticles flocculated onto said primary support; and

    d. contacting a sample suspected of containing a binder or ligand complementary to the ligand or binder attached on the affinity support of step c to separate said binder or ligand from the sample.

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