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Apparatus for measuring spatial distribution of fluorescence on a substrate

  • US 5,424,841 A
  • Filed: 05/28/1993
  • Issued: 06/13/1995
  • Est. Priority Date: 05/28/1993
  • Status: Expired due to Fees
First Claim
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1. Apparatus for measuring the spatial distribution of fluorescence on a substrate having multiple fluorophores each having a different characteristic wavelength comprising:

  • scanning means for directing a radiant excitation beam at known locations on a substrate containing a plurality of fluorophore targets, said locations defining a scanning path;

    collecting means for simultaneously collecting radiation from the scanning path;

    detecting means positioned to receive radiation from said collecting means, said detecting means producing a signal corresponding to the intensity of said collected radiation;

    first filter means positioned between said collecting means and said scanning path, said first filter means selectively rejecting background wavelengths contained in said excitation beam;

    second filter means positioned in the radiation path between said collecting means and said detecting means, said second filter means selectively rejecting background wavelengths contained in said excitation beam; and

    third filter means for interchangeably positioning one of a plurality of band pass filters between said second filter means and said detecting means, each of said band pass filters selectively transmitting a different band of wavelengths, each of said bands being associated with a different fluorophore,whereby the distribution of fluorescence on the substrate at selected wavelengths at said known locations can be measured.

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