DNA encoding cytokine-induced protein, TSG-14

• US 5,426,181 A
• Filed: 08/14/1992
• Issued: 06/20/1995
• Est. Priority Date: 01/14/1991
• Status: Expired due to Term

• Current Assignee: New York University
• Original Assignee: New York University
• Inventors: Lee, Tae H., Lee, Gene W., Vilcek, Jan
• Primary Examiner(s): Draper, Garnette D.
• Assistant Examiner(s): KEMMERER, ELIZABETH
• Application Number:
  US07/929,580
• Time in Patent Office: 1,040 Days
• Field of Search: 435/69.1, 435/69.5, 435/240.1, 435/252.3, 435/243, 536/23.1, 536/23.5
• US Class Current: 536/23.5
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/4718 Cytokine-induced proteins
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C07K 2319/75 containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Abstract

Pleiotropic pro-inflammatory cytokines, such as TNF and IL-1, induce expression of a polypeptide molecule, termed TSG-14, in connective tissue cells. The TSG-14 polypeptide and functional derivatives thereof, DNA coding therefor, expression vehicles, such as a plasmids, and host cells transformed or transfected with the DNA molecule, and methods for producing the polypeptide and the DNA are provided. Antibodies specific for the TSG-14 polypeptide are disclosed, as is a method for detecting the presence of TSG-14 polypeptide in a biological sample, using the antibody or another molecule capable of binding to TSG-14 such as hyaluronic acid. A method for detecting the presence of nucleic acid encoding a normal or mutant TSG-14 polypeptide, a method for measuring induction of expression of TSG-14 in a cell using either nucleic acid hybridization or immunoassay, a method for identifying a compound capable of inducing the expression of TSG-14 in a cell, and a method for measuring the ability of a cell to respond to TNF are also provided.

Claims

1. An isolated DNA molecule encoding a tumor-necrosis factor-stimulated gene-14 (TSG-14) polypeptide, comprising a DNA encoding the amino acid sequence of FIG. 9A (SEQ ID NO:

4).

2. An isolated DNA molecule according to claim 1, wherein said DNA is derived from the group consisting of cDNA and genomic DNA.

3. An isolated DNA molecule according to claim 1, further comprising an expression vehicle.

4. An isolated DNA molecule according to claim 3, wherein said vehicle further comprises a plasmid.

5. A prokaryotic host cell transformed with an isolated DNA molecule according to claim 1.

6. A host according to claim 5, wherein said host is a bacterium.

7. A eukaryotic host cell transformed with an isolated DNA molecule according to claim 1.

8. A host according to claim 7, wherein said host is selected from a yeast cell and a mammalian cell.