Method of making and amperometric electrodes

• US 5,429,735 A
• Filed: 06/27/1994
• Issued: 07/04/1995
• Est. Priority Date: 06/27/1994
• Status: Expired due to Term

• Current Assignee: Baayer Corporation
• Original Assignee: Miles, Inc. (Bayer AG)
• Inventors: Musho, Matthew K., Johnson, Larry D., Murray, Alison J.
• Primary Examiner(s): Niebling: John
• Assistant Examiner(s): Bell, Bruce F.
• Application Number:
  US08/265,913
• Time in Patent Office: 372 Days
• Field of Search: 204/403, 204/412, 204/402, 204/298.01, 204/192.1, 204/157.43, 204/177, 204/418, 435/817
• US Class Current: 204/403.11
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A method of making and an amperometric electrode are provided. An electrode carbon ink is applied to a polymer substrate to form a working electrode. The substrate carrying the working electrode is placed in a gas plasma cleaner, such as an oxygen or nitrogen plasma, to clean the working electrode. A high radio frequency signal excites the gas plasma for a short exposure time in a range between 10 seconds and 30 seconds. Then a reagent layer is deposited to the plasma treated working electrode.

Claims

1. A method of making an amperometric electrode comprising the steps of:

providing a substrate;

applying an electrode carbon ink to said substrate to form a working electrode;

said electrode carbon ink containing set amounts of graphite and carbon black;

cleaning said working electrode utilizing a gas plasma;

said gas plasma is a nitrogen gas plasma or an oxygen gas plasma; and

depositing a reagent layer to said working electrode after said cleaning step.

2. A method of making an amperometric electrode as recited in claim 1 wherein said step of providing a substrate includes the step of providing a polymer substrate.

3. A method of making an amperometric electrode as recited in claim 1 wherein said step of applying an electrode carbon ink to said substrate includes the steps of screen printing said electrode carbon ink to said substrate to form said working electrode;

and thermally drying said screen printed working electrode.

4. A method of making an amperometric electrode as recited in claim 1 wherein said step of applying said electrode carbon ink containing set amounts of graphite and carbon black includes applying an electrode carbon ink containing approximately 18% graphite and 6% carbon black.

5. A method of making an amperometric electrode as recited in claim 1 wherein said step of cleaning said working electrode utilizing a gas plasma includes the steps of:

placing said substrate carrying said working electrode in a chamber;

evacuating said chamber in a range between 0.1 to 0.2 Torr;

backfilling said evacuated chamber with a gas to an operating pressure in a range between 0.3 to 0.5 Torr; and

exciting said gas with a radio frequency (RF) signal.

6. A method of making an amperometric electrode as recited in claim 5 wherein said step of exciting said gas with a radio frequency (RF) signal includes the steps of:

identifying said operating pressure stabilized at a set pressure; and

applying said radio frequency (RF) signal having a set frequency of about 13.56 Mhz with a set power level of 20 to 25 watts.

7. A method of making an amperometric electrode as recited in claim 5 wherein said step of exciting said gas with a radio frequency (RF) signal includes the step of applying a radio frequency (RF) signal having a frequency of about 13.56 Mhz for a selected time period in a range between 10 seconds and 30 seconds.

8. A method of making an amperometric electrode as recited in claim 1 wherein said step of depositing a reagent layer to said working electrode after said cleaning step includes the step of depositing a layer containing an enzyme and an electron transfer agent over said cleaned working electrode.

9. A method of making an amperometric electrode as recited in claim 1 wherein the amperometric electrode is used for a glucose biosensor and wherein said step of depositing a reagent layer to said working electrode after said cleaning step includes the steps of depositing a layer containing a glucose oxidase.

10. A method of making an amperometric electrode as recited in claim 1 wherein said step of providing a substrate includes providing a substrate formed of an electrical insulative, thermoplastic material.

11. An amperometric electrode comprising:

an electrical insulative substrate formed of thermoplastic material;

a working electrode carried by said substrate;

said working electrode is formed by an electrode carbon ink containing graphite and carbon black;

said working electrode is cleaned by a gas plasma;

said gas plasma is a nitrogen gas plasma or an oxygen gas plasma; and

a reagent layer deposited to said gas plasma treated working electrode.

12. An amperometric electrode as recited in claim 11 wherein said working electrode is formed by said electrode carbon ink containing approximately 18% graphite and 6% carbon black.

13. An amperometric electrode as recited in claim 11 wherein said working electrode is formed by screen printing said electrode carbon ink onto said electrical insulative substrate.

14. An amperometric electrode as recited in claim 11 wherein said working electrode is treated by said gas plasma by exciting said gas with a radio frequency (RF) signal having a frequency of about 13.56 Mhz.

15. An amperometric electrode as recited in claim 11 wherein said working electrode is treated by said gas plasma by exciting said gas with a radio frequency (RF) signal having a set frequency of about 13.56 Mhz with a predetermined power level of 20 to 25 watts.