Antigen/anti-antigen cleavage
First Claim
1. A method of cleaving an antigen/anti-antigen or hapten/anti-hapten linkage joining two particles comprising binding to said anti-antigen or anti-hapten of said linkage a secondary antibody or antibody fragment that specifically binds to one or more sites in said anti-antigen or anti-hapten which are not the antigen-binding or hapten-binding sites and is effective upon binding Said one or more sites to cleave said linkage.
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Accused Products
Abstract
The present invention provides a method of cleaving an antigen/anti-antigen or hapten/anti-hapten linkage joining two particles comprising reacting said linkage with a secondary antibody, or fragment thereof, binding to said anti-antigen or anti-hapten, and a kit for performing such a method. The method of the invention has particular utility in the separation of cells.
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Citations
13 Claims
- 1. A method of cleaving an antigen/anti-antigen or hapten/anti-hapten linkage joining two particles comprising binding to said anti-antigen or anti-hapten of said linkage a secondary antibody or antibody fragment that specifically binds to one or more sites in said anti-antigen or anti-hapten which are not the antigen-binding or hapten-binding sites and is effective upon binding Said one or more sites to cleave said linkage.
- 10. A method of positively isolating target cells from a mixed population of cells comprising binding an anti-antigen attached to magnetic particles to antigen on said target cells, said anti-antigen being attached to magnetic particles before or after binding to said target cells, thereby binding said particles to said target cells, isolating said magnetic particles and bound target cells from the mixed population of cells by magnetic aggregation, releasing the target cells from said magnetic particles by adding under conditions effective for binding to occur a secondary antibody or antibody fragment that specifically binds to one or more sites in said anti-antigen which are not the antigen-binding sites and that is effective upon binding said one or more sites to cleave the binding between said target cells and said anti-antigen, thereby isolating the released target cells from said mixed population.
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12. A kit comprising:
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(i) a particulate solid support carrying an anti-antigen or anti-hapten specific for a target antigen or hapten; (ii) a secondary antibody or antibody fragment that specifically binds to one Or more sites in said anti-antigen or anti-hapten which are not the antigen-binding or hapten-binding sites and is effective upon binding said one or more sites to cleave the antigen/anti-antigen or hapten/anti-hapten linkage formed upon binding of said antigen to Said anti-antigen or Said hapten to said anti-hapten. - View Dependent Claims (13)
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Specification