Quantitative analysis of glycosylated hemoglobin by immunocappillary electrophoresis
First Claim
Patent Images
1. A method for determining the content of a first form of hemoglobin in a sample that also contains at least one other form of hemoglobin, the method comprising the steps of:
- (a) adding to a sample containing a first form of hemoglobin and at least one other form of hemoglobin, a macromolecular specific binding partner for the first form of hemoglobin, the specific binding partner specifically binding the first form to yield a complex and not binding to the other forms of hemoglobin in the sample;
(b) subjecting the sample, after step (a), to capillary electrophoresis to generate an electropherogram that separates the complex from the other forms of hemoglobin in the sample; and
(c) determining the content of the first form of hemoglobin in the sample from the electropherogram.
1 Assignment
0 Petitions
Accused Products
Abstract
A method useful for determining the content of a first hemoglobin in a blood sample which also contains other forms of hemoglobin is based on capillary electrophoresis. In the method, a specific binding partner to the first form of hemoglobin is added to the sample, and the sample is then subjected to capillary electrophoresis. The method is particularly suited for the determination of the percentage of Hb A1c in a blood sample using anti-Hb A1c antibody.
41 Citations
25 Claims
-
1. A method for determining the content of a first form of hemoglobin in a sample that also contains at least one other form of hemoglobin, the method comprising the steps of:
-
(a) adding to a sample containing a first form of hemoglobin and at least one other form of hemoglobin, a macromolecular specific binding partner for the first form of hemoglobin, the specific binding partner specifically binding the first form to yield a complex and not binding to the other forms of hemoglobin in the sample; (b) subjecting the sample, after step (a), to capillary electrophoresis to generate an electropherogram that separates the complex from the other forms of hemoglobin in the sample; and (c) determining the content of the first form of hemoglobin in the sample from the electropherogram. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
-
-
10. A method for determining the Hb A1c content of a sample that also contains at least one other form of hemoglobin, the method comprising the steps of:
-
(a) adding to a sample containing Hb A1c and at least one other form of hemoglobin, an antibody specific binding partner for the Hb A1c, the antibody specifically binding Hb A1c to yield a complex, the antibody not binding to the other forms of hemoglobin in the sample; (b) subjecting the sample, after step (a), to capillary electrophoresis utilizing a buffer to generate an electropherogram that separates the complex from the other forms of hemoglobin in the sample, the buffer comprising; (i) water; (ii) boric acid in an amount of from 50 to 150 mM; (iii) a borate salt in an amount of from 15 to 35 mM; (iv) a sodium or potassium salt where the anion is selected from the group consisting of sulfate, chloride, fluoride, iodide, and bromide ions, the amount of sodium or potassium salt being from 50 to 100 mM; and (v) sufficient base compound that the buffer has a pH of from 8 to 10; and (c) determining the quantity of Hb A1c from the electropherogram. - View Dependent Claims (11, 12, 13, 14, 15)
-
-
16. A method for determining the Hb A1c content of a sample that also contains at least one other form of hemoglobin, the method comprising the steps of:
-
(a) adding to a sample containing Hb A1c and at least one other form of hemoglobin, an antibody specific binding partner for the Hb A1c, the antibody specifically binding Hb A1c to yield a complex, the antibody not binding to the other forms of hemoglobin in the sample; (b) subjecting the sample, after step (a), to capillary electrophoresis utilizing a buffer, to generate an electropherogram that separates the complex from the other forms of hemoglobin in the sample, the buffer comprising; (i) water; (ii) boric acid in an amount of about 75 mM; (iii) borax in an amount of about 25 mM; (iv) sodium chloride in an amount of about 75 mM; and (v) sufficient sodium hydroxide that the buffer has a pH of about 8.8; and (c) determining the quantity of Hb A1c from the electropherogram.
-
-
17. A method for determining the content of a first form of hemoglobin in a sample that also contains at least one other form of hemoglobin, the method comprising the steps of:
-
(a) adding to a sample containing a first form of hemoglobin and at least one other form of hemoglobin, a macromolecular specific binding partner for the first form of hemoglobin, the specific binding partner specifically binding the first form to yield a complex, the antibody not binding to the other forms of hemoglobin in the sample; (b) subjecting the sample, after step (a), to capillary electrophoresis to generate an electropherogram that separates the complex from the other forms of hemoglobin in the sample; and (c) determining the content of the first form of hemoglobin in the sample by measuring light absorbance at a wavelength at which hemoglobin significantly absorbs light and at which the macromolecular specific binding partner does not substantially absorb light. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25)
-
Specification