Method for preservation of biological tissue
First Claim
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1. A method for preparing a preserved biological tissue which comprises:
- (a) treating a biological tissue containing water with an aqueous solution consisting essentially of a compound selected from the group consisting of an alkali metal salt, an alkaline earth metal salt, a base and mixtures thereof and then rinsing the biological tissue with water;
(b) freezing the treated biological tissue containing water without use of an intermediary solvent, then;
(c) providing a first vacuum in a chamber containing a support means for the frozen tissue to remove the frozen water from the tissue and to provide a freeze-dried tissue;
(d) immersing the freeze-dried tissue in a non-flammable degreasing solvent to remove lipids from the tissue and to impregnate the tissue with the solvent and then removing the tissue from the solvent;
(e) impregnating the freeze-dried tissue in a container with a silicone polymer precursor and a curing agent for curing the silicone polymer precursor by providing a second vacuum in a chamber containing the container, wherein the silicone polymer precursor and the curing agent impregnate the specimen and the solvent is removed from the tissue to provide an impregnated tissue; and
(f) curing the polymer precursor with the curing agent in the impregnated tissue to form a transparent cured silicone polymer and thus provide the preserved biological tissue.
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Accused Products
Abstract
A method for silicone polymer impregnation and curing in tissue to preserve the tissue. The tissue is frozen without use of an intermediary solvent and then freeze-dried using a first vacuum. The tissue is then immersed in a non-flammable degreasing solvent, particularly 1,1,1-trichloroethane to remove lipids and the like from the tissue. The tissue is then impregnated with a silicone polymer precursor and a curing agent, and then cured under a second vacuum in a chamber (23). The impregnated polymer silicone precursor is then cured in the tissue. The tissue is preferably an anatomical specimen.
43 Citations
28 Claims
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1. A method for preparing a preserved biological tissue which comprises:
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(a) treating a biological tissue containing water with an aqueous solution consisting essentially of a compound selected from the group consisting of an alkali metal salt, an alkaline earth metal salt, a base and mixtures thereof and then rinsing the biological tissue with water; (b) freezing the treated biological tissue containing water without use of an intermediary solvent, then; (c) providing a first vacuum in a chamber containing a support means for the frozen tissue to remove the frozen water from the tissue and to provide a freeze-dried tissue; (d) immersing the freeze-dried tissue in a non-flammable degreasing solvent to remove lipids from the tissue and to impregnate the tissue with the solvent and then removing the tissue from the solvent; (e) impregnating the freeze-dried tissue in a container with a silicone polymer precursor and a curing agent for curing the silicone polymer precursor by providing a second vacuum in a chamber containing the container, wherein the silicone polymer precursor and the curing agent impregnate the specimen and the solvent is removed from the tissue to provide an impregnated tissue; and (f) curing the polymer precursor with the curing agent in the impregnated tissue to form a transparent cured silicone polymer and thus provide the preserved biological tissue.
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2. A method for preparing a preserved biological tissue which comprises:
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(a) treating a biological tissue with an aqueous solution consisting essentially of a compound selected from the group consisting of an alkali metal salt, an alkaline earth metal salt, a base and mixtures thereof and then rinsing the biological tissue with water; (b) freezing a biological tissue containing water without use of an intermediary solvent, then; (c) providing a first vacuum in a chamber containing a support means for the frozen tissue to remove the frozen water from the tissue and to provide a freeze-dried tissue; (d) immersing the freeze-dried tissue in 1,1,1-trichloroethane to remove lipids from the tissue and to impregnate the tissue with the 1,1,1-trichloroethane and then removing the tissue from the 1,1,1-trichloroethane; (e) impregnating the freeze-dried tissue in a container with a silicone polymer precursor and a curing agent for curing the silicone polymer precursor by providing a second vacuum in a chamber containing the container, wherein the silicone polymer precursor and impregnate the specimen and the 1,1,1-trichloroethane is removed from the tissue to provide an impregnated tissue; and (f) curing the polymer precursor with the curing agent to form a transparent cured silicone polymer and thus provide the preserved biological tissue. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method for preparing a preserved biological tissue which comprises:
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(a) freezing a biological tissue containing water without using an intermediary solvent, then; (b) providing a first vacuum in a chamber containing a support means for the frozen tissue to remove the frozen water from the tissue and to provide a freeze-dried tissue; (c) immersing the freeze-dried tissue in 1,1,1-trichloroethane to remove lipids from the tissue and to impregnate the tissue with the 1,1,1-trichloroethane and then removing the tissue from the 1,1,1-trichloroethane; (d) impregnating the freeze-dried tissue in a container with a silicone polymer precursor and a curing agent for curing the silicone polymer precursor by providing a second vacuum in a chamber containing the container, wherein the silicone polymer precursor and the curing agent impregnate the specimen and the 1,1,1-trichloroethane is removed from the tissue to provide an impregnated tissue wherein the vacuum is cycled at least once to remove any air entrapped in the tissue or the silicone polymer precursor; and (e) curing the polymer precursor with the curing agent to form a transparent cured silicone polymer and thus provide the preserved biological tissue. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28)
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Specification