Bisulfite-based tissue fixative
First Claim
1. A tissue fixative comprising an acidic buffer having a pH of from about 4 to about 5 and, as the sole fixative agent, a bisulfite salt in a concentration of from about 20 mM to about 250 mM.
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Abstract
The present invention provides a bisulfite-based tissue fixative that improves morphology, antigen retention, and antigenicity. The fixative is suitable for use with sections of frozen tissue, cytology preparations (including touch preparations and blood smears) and other specimens used for immunohistochemical analysis. The fixative comprises an acidic buffer and a bisulfite salt in an effective amount for tissue fixation. In a preferred embodiment, the tissue fixative comprises a bisulfite salt in a concentration of from about 0.01 to about 0.25M, preferably, 0.02 to 0.25M, in an acidic buffer having a pH of from about 2 to about 6.
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Citations
9 Claims
- 1. A tissue fixative comprising an acidic buffer having a pH of from about 4 to about 5 and, as the sole fixative agent, a bisulfite salt in a concentration of from about 20 mM to about 250 mM.
- 6. A method of fixing tissue comprising contacting said tissue with a tissue fixative comprising an acidic buffer having a pH of from about 4 to about 5 and, as the sole fixative agent, a bisulfite salt in an effective amount for tissue fixation.
Specification