Method for irradiating cells

• US 5,433,738 A
• Filed: 01/25/1994
• Issued: 07/18/1995
• Est. Priority Date: 07/12/1989
• Status: Expired due to Fees

• Current Assignee: Randy L. Stinson
• Original Assignee: Randy L. Stinson
• Inventors: Stinson, Randy L.
• Primary Examiner(s): Graham, Mark S.
• Application Number:
  US08/186,239
• Time in Patent Office: 539 Days
• Field of Search: 128/395-398, 128/480, 607/88-94, 604/4, 250/432 R, 250/455.1, 250/435, 250/436, 250/438, 250/43, 250/437, 422/372, 422/24, 422/44, 435/284
• US Class Current: 607/92
• CPC Class Codes: A61L 2/0011 using physical methods
• CPC Class Codes: A61L 2/10 Ultraviolet radiation
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

An apparatus for irradiating cells with ultraviolet light including an ultraviolet light source and an outer cylinder that surrounds the ultraviolet light source. Hollow tubing is helically wrapped around the outer peripheral surface of the outer cylinder. The hollow tubing is adapted to transport suspended cells over the outer surface of the outer cylinder so that the cells can be irradiated by the ultraviolet light source. An inner cylinder can be positioned inside the outer cylinder, between the ultraviolet light source and the outer cylinder. The apparatus can include an arrangement for ventilating the apparatus during use in order to maintain a substantially constant temperature.

Claims

1. A method of irradiating potential transplant cells with ultraviolet light through use of an apparatus comprising the steps of:

feeding a cell suspension into an inlet end of a transport means for transporting and guiding the cell suspension;

transporting and guiding the cell suspension over at least a portion of an outer peripheral surface of an outer cylinder;

irradiating the suspended cells with ultraviolet light from an ultraviolet light source that is positioned inside the outer cylinder;

ventilating the apparatus by blowing air into a space located between an outer surface of the ultraviolet light source and an inner surface of an inner cylinder positioned between the outer cylinder and the ultraviolet light source, permitting the air to flow into a space located between an inner surface of the outer cylinder and outer surface of the inner cylinder, and permitting the air to exit the apparatus; and

collecting the cell suspension after irradiation in a collection reservoir as the cell suspension exits an outlet of the transport means.

2. The method in accordance with claim 1, further including the step of filtering the ultraviolet light from the ultraviolet light source so that the suspended cells are not irradiated by ultraviolet light having a wavelength less than about 280 nanometers.

3. The method in accordance with claim 1, wherein the step of irradiating the cells with ultraviolet light includes irradiating the cells with ultraviolet light having a wavelength not less than about 280 nanometers.

4. A method of irradiating potential transplant cells with ultraviolet light through use of an apparatus in order to alter antigen expression and recognition of the cells comprising the steps of:

feeding a cell suspension into an inlet end of a helically arranged transport means for transporting and guiding the cell suspension;

helically transporting and guiding the cell suspension through the transport means over at least a portion of an outer peripheral surface of an outer cylinder, the cell suspension being transported and guided through the transport means which is separate from and encircles the outer peripheral surface of the outer cylinder;

altering antigen expression and recognition of the cells by irradiating the suspended cells with ultraviolet light from an ultraviolet light source that is positioned inside the outer cylinder, said cells being irradiated with ultraviolet light having a wavelength not less than about 280 nanometers; and

introducing the irradiated cells whose antigen expression and recognition has been altered into a living recipient.

5. The method in accordance with claim 4, wherein the ultraviolet light is filtered so that the cells are not subjected to ultraviolet light having a wavelength greater than about 320 nanometers.

6. The method in accordance with claim 4, and further including the step of ventilating the apparatus by blowing air into a space located between an outer surface of the ultraviolet light source and an inner surface of an inner cylinder positioned between the outer cylinder and the ultraviolet light source, permitting the air to flow into a space located between an inner surface of the outer cylinder and outer surface of the inner cylinder, and permitting the air to exit the apparatus.

7. The method in accordance with claim 4, wherein the ultraviolet light from the ultraviolet light source is filtered so that the suspended cells are irradiated with ultraviolet light having a wavelength not less than about 280 nanometers.

8. A method of irradiating potential transplant cells with ultraviolet light through use of an apparatus in order to alter antigen expression and recognition of the cells comprising the steps of:

feeding a cell suspension into an inlet end of a helically arranged transport means for transporting and guiding the cell suspension;

helically transporting and guiding the cell suspension through the transport means over at least a portion of an outer peripheral surface of an outer cylinder;

altering antigen expression and recognition of the cells by irradiating the suspended cells with ultraviolet light from an ultraviolet light source positioned inside an inner cylinder which is disposed within and spaced from the outer cylinder, said cells being irradiated with ultraviolet light having a wavelength not less than about 280 nanometers; and

introducing the irradiated cells whose antigen expression and recognition has been altered into a living recipient.

9. The method in accordance with claim 8, further including the steps of permitting the ultraviolet light source to burn for a predetermined continuous period of time prior to feeding the cell suspension into the transport means in order to stabilize the intensity and output of the ultraviolet light source and to permit calibration of the apparatus.

10. The method in accordance with claim 8, wherein said step of irradiating the cells includes irradiating the cells with ultraviolet light having a wavelength not greater than about 320 nanometers.

11. A method of inducing alteration of antigen expression and recognition of cells, comprising:

providing cells from a donor source;

subjecting said cells to ultraviolet radiation while said cells are being conveyed through a helically arranged conveying means to alter antigen expression and recognition of the cells to the extent necessary to change immune response to the cells, said cells being subjected to ultraviolet irradiation by subjecting the cells to ultraviolet light having a wavelength not less than 280 nanometers, said cells being conveyed in a helical path through the conveying means which is separate from and encircles an outer peripheral surface of an outer cylinder; and

introducing into a living recipient the cells whose antigen expression has been altered through ultraviolet irradiation in order to modify antigen recognition of the transplanted cells and thereby change immune response in the living recipient.

12. The method according to claim 11, wherein said step of subjecting the cells to ultraviolet irradiation includes subjecting the cells to ultraviolet light that has been filtered to remove ultraviolet light having a wavelength greater than about 320 nanometers.

13. The method according to claim 11, wherein said step of subjecting the cells to ultraviolet irradiation includes subjecting the cells to ultraviolet light that has been filtered to remove ultraviolet light having a wavelength less than about 280 nanometers.

14. A method of inducing alteration of antigen expression and recognition of cells, comprising:

providing cells from a donor source;

subjecting said cells to ultraviolet radiation while said cells are being conveyed through conveying means to alter antigen expression and recognition to the extent necessary to change immune response to the cells, said cells being subjected to ultraviolet irradiation by subjecting the cells to ultraviolet light having a wavelength not less than 280 nanometers, the cells being conveyed through conveying means positioned exteriorly of an outer cylinder and being subjected to ultraviolet radiation from an ultraviolet radiation source positioned inside an inner cylinder which is disposed within and spaced from the outer cylinder; and

introducing into a living recipient the cells whose antigen expression has been altered through ultraviolet irradiation in order to modify antigen recognition of the transplanted cells and thereby change immune response in the living recipient.

15. The method according to claim 14, wherein said conveying of the cells through conveying means includes conveying the cells through helically wound tubing while being subjected to the ultraviolet irradiation.

16. The method in accordance with claim 14, wherein said step of irradiating the cells includes irradiating the cells with ultraviolet light having a wavelength not greater than about 320 nanometers.