Controlled release systems and low dose androgens

• US 5,434,146 A
• Filed: 06/24/1992
• Issued: 07/18/1995
• Est. Priority Date: 06/28/1991
• Status: Expired due to Fees

• Current Assignee: Endorecherche
• Original Assignee: Endorecherche
• Inventors: Labrie, Fernand, Lepage, Martin
• Primary Examiner(s): Nutter, Nathan M.
• Application Number:
  US07/900,817
• Time in Patent Office: 1,119 Days
• Field of Search: 514/169, 514/170, 514/177, 514/179, 523/113, 424/422
• US Class Current: 514/169
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/565 not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol
• CPC Class Codes: A61K 31/569 substituted in position 17 alpha, e.g. ethisterone
• CPC Class Codes: A61K 31/57 substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone
• CPC Class Codes: A61K 31/575 substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol
• CPC Class Codes: A61K 9/1647 Polyesters, e.g. poly(lactide-co-glycolide)
• CPC Class Codes: A61K 9/1694 resulting in granules or microspheres of the matrix type containing more than 5% of excipient
• CPC Class Codes: A61P 15/00 Drugs for genital or sexual disorders for disorders of sex hormones A61P5/24; Contraceptives
• CPC Class Codes: A61P 15/18 Feminine contraceptives
• CPC Class Codes: A61P 19/10 for osteoporosis
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: Y10T 428/2982 Particulate matter [e.g., sphere, flake, etc.]

Abstract

Methods of treatment and prevention of estrogen-related diseases, and of fertility control, include low dose (e.g. less than 50 nanomolaR serum concentration) administration of certain anabolic steroids, progestins and other substantially non-masculinizing androgenic compounds. Sustained release formulations substantially free of organic solvent, and sustained release formulations for maintaining low serum levels of androgen are disclosed.

Claims

1. A method of treating or preventing breast cancer by administering to a patient in need of such treatment or prevention, a therapeutically effective amount of sustained release particles, with or without additional pharmaceutical carriers or diluents, said particles comprising an androgenic steroid dispersed within a sustained-release binder which is biocompatible with human tissue and which undergoes biodegradation in the body into biocompatible metabolic products, wherein said particles are capable, under standard conditions, of releasing said androgenic steroid during and as a result of said biodegradation of said binder at a rate and duration which maintains circulating serum levels of said androgenic steroid between 1.0 and 50.0 nanomoles per liter during a time period beginning 48 hours after administration and ending at least 28 days after administration, wherein said androgenic steroid has a Ki value of less than about 2 ×

10^-8 M for the androgen receptor, and an androgen receptor-mediated inhibitory effect on the growth of human breast cancer ZR-75-1 cells which reaches half-maximal value at a concentration below 3.0 nanomoles per liter, and no visible masculinizing activity.

2. The method of claim 1 wherein said binder is a polymeric matrix comprising copolymeric or homopolymeric polyesters having hydrolyzable ester linkages.

3. The method of claim 1 wherein said binder is a polylactide.

4. The method of claim 1 wherein said binder is a copolymer of lactide and at least one monomer selected from the group consisting of glycolide and caprolactone.

5. The method of claim 4 wherein said copolymer is between 50 and 90 mol-% lactide.

6. The method of claim 1 wherein the ratio of said androgenic steroid to said binder is between 1:

4 and 7;

3 (by weight).

7. The method of claim 1 wherein said binder is selected from the group consisting of poly(ε

-caprolactone), poly(ε

-caprolactone-CO-lactide acid), poly(ε

-caprolactone-CO-glycolide), poly(β

-hydroxy butyrate), poly(alkyl-2-cyanoacrilate), hydrogels such as poly(hydroxyethyl methacrylate), polyamides, poly(amino acides) (i.e. L-leucine, glutamic acid, L-aspartic acid and the like), poly (ester urea), poly (2-hydroxyethyl DL-aspartamide), polyacetal polymers, polyorthoesters, polycarbonate, polymaleamides, polysaccharides and copolymers thereof.

8. The method of claim 1 wherein said particles are substantially free of thermal degradation impurities.

9. The method of claim 1 wherein the average particle size is between 5 and 40 μ

m.

10. The method of claim 1 wherein the average particle size is between 9 and 28 μ

m.

11. The pharmaceutical composition of claim 1 wherein said particles are microspheres having an average diameter between 5 and 40 μ

m.

12. The method of claim 1 wherein said particles are microspheres formed by dissolving said binder and said androgenic steroid into an organic solvent to form a solution, and then pouring said solution into water with sufficient stirring to form said microspheres and to assure that said microspheres have an average diameter between 5 and 40 μ

m.

13. The method of claim 1 wherein said androgenic steroid is medroxyprogesterone acetate.

14. The method of claim 1 wherein said androgenic steroid is megestrol acetate.

15. The method of claim 1 wherein at least 90% of said particles are less than 40 microns in size.

16. The method of claim 1 wherein at least 90% of said particles are between 9 and 28 microns in size.