Rhizopus host vector system

• US 5,436,158 A
• Filed: 12/14/1993
• Issued: 07/25/1995
• Est. Priority Date: 10/04/1990
• Status: Expired due to Term

• Current Assignee: Nihon Shokuhin Kako Co., Ltd.
• Original Assignee: Nihon Shokuhin Kako Co., Ltd.
• Inventors: Yanai, Koji, Takagi, Masamichi, Horiuchi, Hiroyuki, Sakaguchi, Kenji
• Primary Examiner(s): Schwartz, Richard A.
• Assistant Examiner(s): CARTER, PHILIP
• Application Number:
  US08/165,881
• Time in Patent Office: 588 Days
• Field of Search: 435/320.1, 435/254.9, 435/69.1
• US Class Current: 435/254.9
• CPC Class Codes: C12N 1/145 Fungal isolates
• CPC Class Codes: C12N 15/80 for fungi
• CPC Class Codes: C12R 2001/845 Rhizopus

Abstract

A host vector system comprising Rhizopus species such as Rhizopus niveus (IFO4810) and a plasmid derived from Phycomycetes such as Mucor, Phycomyces and Absidia wherein the plasmid is contained in a chromosome and/or cytoplasm of the Rhizopus species. The host vector system is suitable for the extracellular production of proteins and enzymes. A process for preparing the host vector system is also provided.

Claims

1. A host vector system for the production of a target protein in Rhizopus said system comprising a Rhizopus species transformed with at least one plasmid derived from Mucor circinelloides wherein said plasmid contains an origin of replication from pLeu4 and encodes said target protein.

2. The host vector system of claim 1, wherein the Rhizopus species is selected from the group consisting of Rhizopus niveus, Rhizopus delemar, Rhizopus javanicus, Rhizopus oligosporus, Rhizopus nigricans and Rhizopus oryzae.

3. The host vector system of claim 2 wherein the Rhizopus species is Rhizopus niveus.

4. The host vector system of claim 3 wherein at least one plasmid is integrated into a chromosome of Rhizopus niveus.

5. The host vector system of claim 3 wherein at least one plasmid is extrachromosomal.

6. The host vector system of claim 3 wherein at least one plasmid is integrated into a chromosome of Rhizopus niveus and at least one plasmid is extrachromosomal.

7. The host vector system of claim 3 wherein the plasmid is pMCL1302, pMCL002,pMA67, pLeu4 or pLeu41.