Support-bound oligonucleotides
First Claim
1. In a method of preparing an oligonucleotide bound to a support bya) attaching to a support a 2'"'"'-deoxynucleoside reagent selected from the group consisting of nucleoside 3'"'"' reagents and nucleoside 5'"'"' reagents which reagent is selected from the group consisting of phosphite, phosphonate, hydrogen phosphonate, phosphonamidite, phosphoramidite and methylphosphonamidite reagents,b) synthesizing on the supported nucleoside an oligonucleotide chain including protecting groups, andc) removing the protecting groups from the oligonucleotide chain,wherein the support carries hydroxyl groups, whereby in step a) the nucleoside becomes attached to the support by a covalent phosphodiester link which is stable to the conditions used in step c),the improvement wherein the groups carried by the support have the formula --ROH, where R is selected from the group consisting of alkoxy, poly(alkoxy) and --(Cn H2n)-- wherein n is at least 3.
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Accused Products
Abstract
A method for synthesizing oligonucleotides by solid-phase methodology wherein the linkage between the oligonucleotide and the solid phase support is a non-labile phosphodiester. The spacer arm between the support and the phosphodiester is a hydrophilic group. The product of this method is an immobilized oligonucleotide which can be used in solid phase hybridization assays.
441 Citations
8 Claims
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1. In a method of preparing an oligonucleotide bound to a support by
a) attaching to a support a 2'"'"'-deoxynucleoside reagent selected from the group consisting of nucleoside 3'"'"' reagents and nucleoside 5'"'"' reagents which reagent is selected from the group consisting of phosphite, phosphonate, hydrogen phosphonate, phosphonamidite, phosphoramidite and methylphosphonamidite reagents, b) synthesizing on the supported nucleoside an oligonucleotide chain including protecting groups, and c) removing the protecting groups from the oligonucleotide chain, wherein the support carries hydroxyl groups, whereby in step a) the nucleoside becomes attached to the support by a covalent phosphodiester link which is stable to the conditions used in step c), the improvement wherein the groups carried by the support have the formula --ROH, where R is selected from the group consisting of alkoxy, poly(alkoxy) and --(Cn H2n)-- wherein n is at least 3.
Specification